Gibco™ DMEM, no glucose

Used as basal medium for supporting the growth of many different mammalian cells. Gibco™ DMEM, No Glucose is used in cell culture processing applications.

Supplier:  Gibco™ 11966025

Catalog No. 11-966-025

Description

• Contains four times the concentration of amino acids and vitamins than the original Eagle's Minimal Essential Medium
• Originally formulated with low glucose (1g/L) and sodium pyruvate, but is often used with higher glucose levels, with or without sodium pyruvate
• Contains no proteins, lipids, or growth factors
• Requires supplementation, commonly with 10% Fetal Bovine Serum (FBS)
• Uses sodium bicarbonate buffer system (3.7g/L), and therefore requires a 5 to 10% CO₂ environment to maintain physiological pH
• Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, cell lines such as HeLa, 293, Cos-7, and PC-12

Order Info

Shipping Condition: Room Temperature

Specifications

• Content And Storage: Storage conditions: 2°C to 8°C (protect from light)
  Shipping conditions: Ambient
  Shelf life: 12 months from date of manufacture
• Cell Type: Primary Fibroblasts, Neurons, Glial Cells, HUVECs, Smooth Muscle Cells
• Concentration: 1 X
• Product Type: DMEM (Dulbecco's Modified Eagle Medium)
• Sterility: Sterile-filtered
• Without Additives: No Glucose, No HEPES, No Sodium Pyruvate
• Manufacturing Quality: cGMP-compliant under the ISO 13485 standard
• Quantity: 500 mL

• Cell Line: HeLa, 293, Cos-7, and PC-12
• Classification: Animal Origin-free
• Form: Liquid
• Serum Level: Standard Serum Supplementation
• With Additives: Glutamine, Phenol Red
• Green Features: Sustainable packaging
• Product Line: Gibco™
• Shipping Condition: Room Temperature

Safety and Handling

ShelfLife : 12 Months From Date of Manufacture

For Research Use Only. Not for use in diagnostic procedures. Class II Device U.S.