Gibco™ DMEM, high glucose, no glutamine, no phenol red

DMEM (Dulbecco's Modified Eagle Medium) is a widely used basal medium for supporting the growth of many different mammalian cells.

Supplier:  Gibco™ 31053028

Catalog No. 31-053-028

Description

DMEM (Dulbecco's Modified Eagle Medium) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12. We offer a variety of DMEM modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

This DMEM is modified as follows:
With: High Glucose
Without: L-glutamine, Phenol Red, Sodium Pyruvate, HEPES

The complete formulation is available.

Using DMEM
DMEM is unique from other media as it contains 4 times the concentration of amino acids and vitamins than the original Eagle's Minimal Essential Medium. DMEM was originally formulated with low glucose (1 g/L) and sodium pyruvate, but is often used with higher glucose levels, with or without sodium pyruvate. DMEM contains no proteins, lipids, or growth factors. Therefore, DMEM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). DMEM uses a sodium bicarbonate buffer system (3.7 g/L), and therefore requires a 5–10% CO₂ environment to maintain physiological pH.

Order Info

Shipping Condition: Room Temperature

Compliance

DMEM is manufactured at a cGMP-compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.

Specifications

• Content And Storage: Storage conditions: 2-8° C. Protect from light
  Shipping conditions: Ambient
  Shelf life: 12 months from date of manufacture
• Cell Type: Primary Fibroblasts, Neurons, Glial Cells, HUVECs, Smooth Muscle Cells
• Concentration: 1 X
• Product Type: DMEM (Dulbecco's Modified Eagle Medium)
• Sterility: Sterile-filtered
• Without Additives: No Glutamine, No HEPES, No Phenol Red, No Sodium Pyruvate
• Green Features: Sustainable packaging
• Product Line: Gibco™
• Shipping Condition: Room Temperature

• Cell Line: HeLa, 293, Cos-7, and PC-12
• Classification: Animal Origin-free
• Form: Liquid
• Serum Level: Standard Serum Supplementation
• With Additives: High Glucose
• Glucose Concentration: 4500 mg/L
• Manufacturing Quality: cGMP-compliant under the ISO 13485 standard
• Quantity: 500 mL

Safety and Handling

ShelfLife : 12 Months From Date of Manufacture

In vitro diagnostic