Gibco™ DMEM, high glucose, GlutaMAX™ Supplement, pyruvate

Used as basal medium for supporting the growth of many different mammalian cells. Gibco™ DMEM, High Glucose, GlutaMAX™ Supplement, Pyruvate is used in human ex vivo tissue and cell culture processing applications.

Catalog Number	Mfr. No.	Quantity	For Use With (Application)
10-569-010	Gibco™ 10569010	500 mL	Mammalian cell culture
10-569-044	Gibco™ 10569044	10 x 500 mL
10-569-069	Gibco™ 10569069	5 L	Antibody and cell culture production, Mammalian cell culture
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10-569-077	Gibco™ 10569077	10 L	Antibody and cell culture production, Mammalian cell culture
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Description

• Contains 4 times the concentration of amino acids and vitamins than the original Eagle's Minimal Essential Medium
• GlutaMAX supplement minimizes toxic ammonia build-up and improves cell viability and growth in easy-to-use format
• Originally formulated with low glucose (1g/L) and sodium pyruvate, but is often used with higher glucose levels, with or without sodium pyruvate
• Contains no proteins, lipids, or growth factors
• Requires supplementation, commonly with 10% Fetal Bovine Serum (FBS)
• Uses sodium bicarbonate buffer system (3.7g/L), and therefore requires a 5 to 10% CO₂ environment to maintain physiological pH
• Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, cell lines such as HeLa, 293, Cos-7, and PC-12

Order Info

Shipping Condition: Room Temperature

Specifications

• Classification: Animal Origin-free
• Sterility: Sterile-filtered
• Without Additives: No HEPES

• Form: Liquid
• With Additives: High Glucose, GlutaMAX, Phenol Red, Sodium Pyruvate

Safety and Handling

ShelfLife : 12 Months From Date of Manufacture

When used as a medical device, Federal law restricts this device to sale by or on the order of a physician. Customers using Gibco DMEM in a manufacturing process, who have a submission with the FDA, may request a letter of authorization from Life Technologies to reference their Type II Drug Master File. For human ex vivo tissue and cell culture processing applications.