Gibco™ Advanced RPMI 1640 Medium

Advanced RPMI (Roswell Park Memorial Institute) 1640 is a widely used basal medium that allows the culture of mammalian cells with reduced Fetal Bovine Serum (FBS) supplementation.

Catalog Number	Mfr. No.	Quantity
12-633-012	Gibco™ 12633012	500 mL
12-633-020	Gibco™ 12633020	10 x 500 mL

Description

Advanced RPMI (Roswell Park Memorial Institute) 1640 is a widely used basal medium that allows the culture of mammalian cells with reduced Fetal Bovine Serum (FBS) supplementation. Compared to classic RPMI 1640, serum supplementation can be reduced by 50-90% with no change in growth rate or morphology.ⁱ Cells successfully cultured in Advanced RPMI 1640, with no adaptation, include Sp2, Vero, Raji, Daudi, and Jurkat.

This Advanced RPMI 1640 is manufactured as follows:
With: Glucose, Non-essential Amino Acids, Sodium Pyruvate, Phenol Red
Without: L-glutamine, HEPES

The complete formulation is available.

Gibco™ Advanced RPMI 1640 is unique from other media due to addition of the following ingredients to allow for serum reduction: ethanolamine, glutathione, ascorbic acid, insulin, transferrin, AlbuMAX™ I lipid-rich bovine serum albumin for cell culture, and the trace elements sodium selenite, ammonium metavanadate, cupric sulfate, and manganous chloride.

Product Use
For Research Use Only. Not intended for animal or human diagnostic or therapeutic use.

Advanced RPMI 1640 requires supplementation with 1-5% Fetal Bovine Serum and 4 mM L-glutamine or GlutaMAX™ supplement. Many cell lines do not require adaptation to this media. The FBS concentration must be optimized for each cell line to obtain maximum serum reduction. Advanced RPMI 1640 uses a sodium bicarbonate buffer system (3.7 g/L) and therefore requires a 5-10% CO₂ environment to maintain physiological pH.

ⁱAdvanced Media: Reduced Sera Solutions. Quest, 2004. 1.3:26-30.

Order Info

Shipping Condition: Room Temperature

Compliance

Gibco Advanced RPMI 1640 is manufactured at a cGMP compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standards.

Specifications

• Content And Storage: Storage conditions: 2-8° C. Protect from light
  Shipping conditions: Ambient
  Shelf life: 12 months from date of manufacture
• Classification: Animal Origin
• Form: Liquid
• Serum Level: Reduced Serum
• With Additives: Glucose, Phenol Red, Sodium Pyruvate, Non Essential Amino Acids (NEAA)
• Green Features: Sustainable packaging
• Product Line: Gibco™

• Cell Line: Sp2, Vero, Raji, Daudi, and Jurkat
• Concentration: 1 X
• Product Type: RPMI 1640 Medium (Roswell Park Memorial Institute 1640 Medium)
• Sterility: Sterile-filtered
• Without Additives: No Glutamine, No HEPES
• Manufacturing Quality: cGMP-compliant under the ISO 13485 standard

Safety and Handling

ShelfLife : 12 Months From Date of Manufacture

For Research Use Only. Not for use in diagnostic procedures.