Agilent BioTek Gen5 Microplate Reader and Imager Software

For use with Imaging Multi-Mode Readers.

Catalog Number	Mfr. No.	ProductTypeSpecs
BTGEN5IPRIM	Agilent BioTek GEN5IPRIME
BTGENSCPRIM	Agilent BioTek GENSECPRIME

Description

• Offers a unique combination of power and ease-of-use that drives productivity and saves time
• Helps transform raw data and images into meaningful results
• Performs high magnification, intracellular image analysis to basic ELISA

Gather and Analyze Data Quickly and Easily

• Quick start with >150 prepared protocols available in Gen5 and online
• Endpoint, kinetic, well area scanning, spectral scanning read modes
• One-button quick export sends data directly to Excel, no need for multiple steps
• Predefined Take3 Micro-Volume Plate protocols for direct quantification of 2µL samples

Explore, Image and Analyze Intra-and Inter-cellular Activity

• Acquire high quality images from 1.25x to 100x with Lionheart FX and up to 60x with Cytation
• Combine image acquisition steps and multi-mode detection steps to rapidly obtain and analyze phenotypic and quantitative data
• Advanced features including image stitching, z-projection and digital phase contrast
• Import image files from other programs for analysis in Gen5

Imaging: Methods and Processes

• Automated cell counting and analysis with image statistics
• Time-lapse movie making with automated image alignment
• Montage capture, image stitching
• Z-stacking and z-projection
• High speed imaging, image binning for fast kinetics

QC and Validation Tools

• Fully validated at Agilent BioTek
• Gen5 Validation Package provides an efficient step-by-step approach to facilitate internal validation
• Compatible with the comprehensive microplate reader IQ/OQ/PQ (Product Qualification Packages) to help speed instrument qualification
• Gen5 IVD and Gen5 IVD Image+ have QC trending capability, security features and validation protocols for convenience and efficiency

Gen5 Image Prime for Cell Imaging

• Offers powerful image acquisition and analysis tool for use with Lionheart FX Automated Cell Imager and Cytation Cell Imaging Multi­Mode Readers
• Cell counting, subpopulation analysis, advanced analysis features
• Multi-mode and imaging steps combined in single protocol allow hit-picking for imaging efficiency, no need to collect unnecessary data
• Image processing and analysis including Z-projection, image stitching, hitpicking and digital phase contrast, plus advanced features including secondary mask, dynamic thresholding and subpopulation analysis
• Built-in movie maker for acquired kinetic imaging sequences
• Fluorescence, brightfield, color brightfield and phase contrast image acquisition
• Import image files from other programs for analysis in Gen5

GxP Compliance with Gen5 Secure

• For regulated laboratory environments, Gen5 Secure offers functionality to help comply with the requirements of 21 CFR Part 11
• Offers imaging control for Lionheart FX Automated Live Cell Imager and Cytation Cell Imaging Multi-Mode Readers
• Includes image processing and analysis including z-projection, image stitching, hitpicking and digital phase contrast
• Advanced features include secondary mask, dynamic thresholding and subpopulation analysis
• Complete reader control and data analysis for all Agilent BioTek microplate readers
• Includes features designed to help laboratories maintain critical GxP requirements including 21 CFR Part 11
• Electronic signature for data verification, approval, authorization and other customizable events
• Data, protocol and system audit trails track and record actions with customizable comments
• Multi-level user permission assignment with highly granular list of protectable functions
• Multiple groups, with multiple users and permissions are definable
• User lockout and reset, password aging are all customizable
• Email notifications for administrator-defined events
• Single sign-on setup available
• Multiple administrators can be assigned

Computer System Requirements

• 64-bit version of Windows 7 or Windows 10 Professional
• Intel Core i5 (4th generation) Processor or higher
• Intel 8 USB Chipset or higher
• 8 GB RAM or greater
• 128 GB Hard Drive space or greater
• Monitor resolution of 1600 x 900
• Keyboard and mouse

Software (Lionheart FX requires Gen5 3.0 or higher)

• Microsoft Internet Explorer v 9.0 or later (for online Help)
• Microsoft Excel for Quick Export and PowerExport: Gen5 3.0: Excel 2007 (32-bit edition only) or Excel 2010 - 2016 (32- and 64-bit editions); Gen5 2.0: Excel 2007 - 2016 (32-bit editions only)

Connectivity

• USB 3 port for camera
• USB 2 or 3 port for instrument

Specifications

• For Use With (Equipment): Imaging Multi-Mode Readers