Molecular Probes™ Fluorescent Protein Labeling Kits

Form stable dye–protein conjugates across the spectrum with any of our 16 available protein labeling kits for use in various fluorescence microscopy applications including flow cytometry, IHC/IF/ICC, FISH, and high content analysis.

Catalog Number	Mfr. No.	Description
A10170	Molecular Probes™ A10170	Alexa Fluor™ 350 Protein Labeling Kit
A10237	Molecular Probes™ A10237	Alexa Fluor™ 546 Protein Labeling Kit
A20174	Molecular Probes™ A20174	Alexa Fluor™ 555 Protein Labeling Kit
A10238	Molecular Probes™ A10238	Alexa Fluor™ 568 Protein Labeling Kit
A10239	Molecular Probes™ A10239	Alexa Fluor™ 594 Protein Labeling Kit
A20171	Molecular Probes™ A20171	Alexa Fluor™ 660 Protein Labeling Kit
A20172	Molecular Probes™ A20172	Alexa Fluor™ 680 Protein Labeling Kit
F10240	Molecular Probes™ F10240	Fluorescein-EX Protein Labeling Kit
O10241	Molecular Probes™ O10241	Oregon Green™ 488 Protein Labeling Kit
50-112-1573	Molecular Probes™ P30012	Pacific Blue™ Protein Labeling Kit
D20655	Molecular Probes™ D20655	DBS-X™ Biotin Protein Labeling Kit

Description

Ready to use in just 90 minutes, our protein labeling kits include an easy-to-use, pre-packed spin column for rapid dye removal and typical recovery greater than 85%. Each kit contains enough reagent for 3–5 protein conjugation reactions. Our kits provide better results due to lower background fluorescence, less nonspecific binding, and easier workflows for protein conjugation with 16 different fluorophores.

• Fluorescently label up to 1 mg of protein per reaction (three reactions per kit)
• Label 0.5–3 mg per reaction with DSB-X Biotin Protein Labeling Kit (five reactions per kit)
• Labeled proteins ready to use in 90 min. (∼15 min. hands-on time)
• Rapidly purify proteins by quickly removing unbound dye using pre-packed spin columns for >85% recovery
• Includes detailed instructions for determining degree of labeling (DOL)

Each protein labeling kit contains everything you need to perform 3-5 separate labeling reactions and purify the resulting conjugates. The reactive dye has either a succinimidyl ester (SE) or a tetrafluorophenyl (TFP) ester moiety that reacts efficiently with primary amines of proteins to form stable dye–protein conjugates. Each of the vials of reactive dye provided in the kit is sufficient for labeling 1 mg of a variety of purified proteins, including growth factors, cytokines, nanobodies, enzymes, cell-adhesion molecules, and antibodies.

Direct labeling with fluorophores allows multiple primary antibodies of the same isotype (derived from the same species) to be used in the same experiment. Stabilizing proteins such as BSA should be removed from the sample before labeling.

The different protein labeling kits
• Blue-fluorescent Alexa Fluor 350—excitation and emission maxima of 346/442 nm
• Green-fluorescent Alexa Fluor 488—excitation and emission maxima of 494/519 nm; excited using a 488 nm argon laser line and detected under standard FITC/Cy2 filters
• Yellow-fluorescent Alexa Fluor 532—excitation and emission maxima of 530/554 nm; excited using a 532 nm Nd:YAG laser line and detected under standard Rhodamine 6G filters
• Orange-fluorescent Alexa Fluor 546—excitation and emission maxima of 554/570 nm; excited using a 543 nm He-Ne laser line and detected under standard TRITC/Cy3 filters
• Orange-fluorescent Alexa Fluor 555—excitation and emission maxima of 555/565 nm; excited using a 543 nm He-Ne laser line and detected under standard TRITC/Cy3 filters
• Orange-red-fluorescent Alexa Fluor 568—excitation and emission maxima of 577/603 nm; excited using a 568 nm Kr laser line and detected under standard Rhodamine Red/Cy3.5 filters
• Red-fluorescent Alexa Fluor 594—excitation and emission maxima of 590/617 nm; excited using a 594 nm Kr or He-Ne laser line and detected under standard Texas Red filters
• Far-red-fluorescent Alexa Fluor 633—excitation and emission maxima of 632/647 nm
• Far-red-fluorescent Alexa Fluor 647—excitation and emission maxima of 650/668 nm; excited using a 633 or 635 nm Kr or He-Ne laser line and detected under standard APC/Cy5 filters.
• Far-red-fluorescent Alexa Fluor 660—excitation and emission maxima of 663/690 nm
• Near-IR-fluorescent Alexa Fluor 680—excitation and emission maxima of 680/700 nm
• Green-fluorescent Fluorescein-EX—excitation and emission maxima of 494/518 nm
• Oregon Green 488—excitation and emission maxima of 496/524 nm
• Pacific Blue—a violet light excitable dye with an excitation and emission maxima of 410/455 nm
• Texas Red-X—excitation and emission maxima of 595/615 nm
• DSB-X Biotin—Conjugates can be reversibly bound to biotin-binding proteins such as streptavidin or avidin. The concentration (mg/mL) of the DSB-X Biotin-labeled antibody preparation can be determined by measuring the absorbance of the dialyzed sample at 280 nm and dividing this value by 1.3 or 1.4 when measured in solution in a cuvette with a 1-cm pathlength. DSB-X Biotin does not absorb significantly at 280 nm.

For labeling smaller amounts of antibodies (~100 µg), we recommend our antibody labeling kits. Please review the protein labeling kit user manual for more in depth information, protocols, molecular weights, and degree of labeling for each dye.

Specifications

• Product Type: Protein Labeling Kit
• Shipping Condition: Room Temperature
• Chemical Reactivity: Amine
• Solubility: DMSO (Dimethylsulfoxide)