Fisher BioReagents™ SurePrep™ RNA/DNA/Protein Purification Kit

Rapid method for the isolation and purification of total RNA, genomic DNA and proteins sequentially from a single sample of cultured animal cells, tissue samples, blood, bacteria, yeast, fungi or plants

Catalog No. BP280250

Includes: Lysis solution (40mL); RNA wash solution (20mL); RNA elution solution (6mL); gDNA wash solution (15mL); gDNA elution buffer (15mL); Protein column regeneration buffer (30mL); Protein column activation and wash buffer (60mL); Protein pH binding buffer (4mL); Protein elution buffer (8mL); Protein neutralizer (2mL); Protein loading dye (2mL); Spin columns (50); Collection tubes (200); Product inserts (1)

Description

Rapid method for the isolation and purification of total RNA, genomic DNA and proteins sequentially from a single sample of cultured animal cells, tissue samples, blood, bacteria, yeast, fungi or plants

The total RNA, genomic DNA and proteins are all column-purified using the same column. This kit is ideal for researchers who are interested in studying the genome, proteome and transcriptome of a single biological sample, such as studies of gene expression including gene silencing experiments or mRNA knockdowns, studies involving biomarker discovery, studies in epigenetics and for characterization of cultured cell lines.

• Fast and easy processing using rapid spin-column format
• All columns for RNA, DNA and protein purification provided
• Sequential isolation of nucleic acids and proteins from a single lysate, no need to split the lysate
• Isolate all sizes of RNA, including large mRNA and rRNA to microRNA (miRNA)
• Isolate high quality total RNA
• Isolate high quality genomic DNA with a molecular weight ≥ 30kb
• High yields of isolated proteins
• No phenol or chloroform extractions

Applications:

Purifed RNA, DNA and protein suitable for assays such as PCR, real-time PCR, reverse transcription PCR, Northern blotting, RNase protection, expression array assays, DNA sequencing, Southern blotting, SNP analysis, SDS-PAGE and Western Blots

Specifications

• Format: Spin Column
• Includes: Lysis solution 40mL, RNA wash solution 20mL, RNA elution solution 6mL, genomic DNA wash solution 15mL, genomic DNA Elution Buffer 15mL, Protein column regeneration Buffer 30mL, Protein column activation and Wash Buffer 60mL, Protein pH Binding Buffer 4mL, Protein Elution Buffer 8mL, Protein neutralizer 2mL, Protein loading dye 2mL, spin columns 50, collection tubes 200, product inserts 1
• Quantity: 50 Preps

• For Use With (Application): PCR, RT-PCR, reverse transcription PCR, northern blotting, RNase protection, expression array assays, DNA sequencing, southern blotting, SNP analysis, SDS-PAGE and western Blots
• No. of Reactions: 50 Preps
• Yield: 15μg RNA HeLa cells (1 x 106 cells), 150μg Protein HeLa cells (1 x 106 cells)