FGF17 Polyclonal Antibody, PeproTech®, Invitrogen™

Rabbit Polyclonal Antibody

Supplier:  Pepro Tech Inc 500P152100UG

Catalog No. 500P152100

Description

AA Sequence of recombinant protein: MTQGENHPSP NFNQYVRDQG AMTDQLSRRQ IREYQLYSRT SGKHVQVTGR RISATAEDGN KFAKLIVETD TFGSRVRIKG AESEKYICMN KRGKLIGKPS GKSKDCVFTE IVLENNYTAF QNARHEGWFM AFTRQGRPRQ ASRSRQNQRE AHFIKRLYQG QLPFPNHAEK QKQFEFVGSA PTRRTKRTRR PQPLT. Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human FGF-17. Anti-Human FGF-17-specific antibody was purified by affinity chromatography employing an immobilized Human FGF-17 matrix. Sandwich ELISA: To detect Human FGF-17 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human FGF-17 (500-P152Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human FGF-17. Western Blot: To detect Human FGF-17 by Western Blot analysis this antibody can be used at a concentration of 0.1- 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human FGF-17 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes including embryonic development cell growth, morphogenesis, tissue repair, tumor growth and invasion. This gene was shown to be prominently expressed in the cerebellum and cortex. The mouse homolog of this gene was localized to specific sites in the midline structures of the forebrain, the midbrain-hindbrain junction, developing skeleton and developing arteries, which suggests a role in central nervous system, bone and vascular development. This gene was referred to as FGF-13 in reference 2, however, its amino acid sequence and chromosomal localization are identical to FGF17.

Specifications

• Antigen: FGF17
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: FGF17
• Gene Alias: FGF; FGF-13; FGF17; FGF-17; Fibroblast growth factor; Fibroblast growth factor 17; HH20; UNQ161/PRO187
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 8822
• Content And Storage: -20°C

• Applications: ELISA, Western Blot
• Concentration: 0.1-1.0 mg/mL
• Formulation: PBS with no preservative
• Gene Accession No.: O60258
• Gene Symbols: FGF17
• Immunogen: E.coli-derived Recombinant Human FGF-17
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human
• Form: Lyophilized