Thermo Scientific™ RevertAid First Strand cDNA Synthesis Kit

Thermo Scientific RevertAid First Strand cDNA Synthesis Kit is a complete system for efficient synthesis of first strand cDNA from RNA templates.

Catalog Number	Mfr. No.	No. of Reactions
FERK1621	Thermo Scientific™ K1621	20 Reactions
FERK1622	Thermo Scientific™ K1622	100 Reactions

Description

Thermo Scientific RevertAid First Strand cDNA Synthesis Kit is a complete system for efficient synthesis of first strand cDNA from RNA templates. The kit uses RevertAid Reverse Transcriptase (RT), a recombinant M-MuLV RT which maintains activity at 42–50°C and is suitable for synthesis of cDNA up to 13 kb. RiboLock RNase Inhibitor, supplied with the kit, effectively protects RNA templates from degradation at temperatures up to 55°C.

The RevertAid First Strand cDNA Synthesis Kit is supplied with both oligo(dT)₁₈ and random hexamer primers. The oligo(dT)₁₈ primer anneals selectively to the poly(A) tail of mRNA. Random hexamer primers do not require the presence of the poly(A) tail; they can therefore be used for transcription of the 5'-end regions of mRNA or cDNA synthesis of RNA species lacking a poly(A) tail (e.g., microRNAs). Gene-specific primers may also be used with the kit.

Radioactively- and non-radioactively-labeled nucleotides can be incorporated into first strand cDNA for use as a probe in hybridization experiments, including microarrays.

Features of the RevertAid First Strand cDNA Synthesis Kit include:
• Full-length first strand cDNA up to 13 kb
• Optimum reaction temperature 42°C
• Complete kit with all components necessary for the RT reaction already included

Applications
• First strand cDNA synthesis for RT-PCR and RT-qPCR
• Construction of full length cDNA libraries
• Antisense RNA synthesis

Specifications

• Content And Storage:

  • RevertAid Reverse Transcriptase
  • RiboLock RNase Inhibitor
  • 5X Reaction Buffer
  • dNTP Mix
  • Oligo(dT)₁₈ Primer
  • Random Hexamer Primer
  • Control GAPDH RNA
  • Forward GAPDH Primer (10 μM)
  • Reverse GAPDH Primer (10 μM)
  • Nuclease-free water
  
  Store at –20°C.
  
  

• Reaction Speed: 60 min.
• Optimal Reaction Temperature: 42°C
• Ribonuclease H Activity: Yes
• For Use With (Application): RT-PCR
• Reaction Format: Separate components
• Size (Final Product): Up to 13 kb

• Format: Kit
• Technique: Reverse Transcription
• Reverse Transcriptase: RevertAid
• Shipping Condition: Dry Ice
• Final Product Type: First-Strand cDNA
• Reagent Type: Reverse Transcription
• Starting Material: RNA

For Research Use Only. Not for use in diagnostic procedures.