Thermo Scientific™ GeneJET PCR Purification Kit

Perform rapid and efficient purification of DNA from PCR and other enzymatic reaction mixtures with this kit.

Supplier:  Thermo Scientific™ K0701

Catalog No. FERK0701

Description

GeneJET PCR Purification Kit utilizes a proprietary silica-based membrane technology in the form of a convenient spin column, eliminating the need for tedious resin manipulations or toxic phenol-chloroform extractions. It effectively removes primers, dNTPs, unincorporated labeled nucleotides, enzymes, and salts from PCR and other reaction mixtures.

The kit can be used for purification of DNA fragments from 25  bp to 20 kb with recovery rates up to 100%. Each GeneJET purification column has a total binding capacity of up to 25 µg of DNA, and the entire procedure takes 5 minutes. The purified DNA can be used in common downstream applications, such as sequencing, restriction digestion, labeling, ligation, cloning, in vitro transcription, blotting or in situ hybridization.

The kit utilizes a proprietary silica-based membrane technology in the form of a convenient spin column, eliminating the need for tedious resin manipulations or toxic phenol-chloroform extractions. The GeneJET PCR Purification Kit effectively removes primers, dNTPs, unincorporated labeled nucleotides, enzymes and salts from PCR and other reaction mixtures. The kit can be used for purification of DNA fragments from 25 bp to 20kb with recovery rates up to 100%. Each GeneJET purification column has a total binding capacity of up to 25μg of DNA and the entire procedure takes just 5 min. The purified DNA can be used in common downstream applications such as sequencing, restriction digestion, labeling, ligation, cloning, in vitro transcription, blotting or in situ hybridization.

• Highly efficient: 90 to 100% recoveries in the range of 100 bp—10kb

• Fast: procedure takes only 5 minutes

• Convenient: spin columns are capped and assembled with collection tubes

Recommended for:

Fast and efficient purification of DNA fragments ideal for use in all conventional molecular biology procedures including:—FastDigest or conventional restriction digestion;—automated fluorescent or radioactive sequencing

Specifications

• Format: Spin Column
• Product Line: GeneJET
• Quantity: 50 Preps
• Target: DNA from Enzymatic Reactions (e.g. PCR)
• Isolation Technology: Spin Column
• Sample Type: dsDNA

• High-throughput Compatibility: Not High-throughput Compatible (Manual)
• Product Type: PCR Purification Kit
• Run Time: 5 min
• For Use With (Application): Real Time PCR (qPCR), Cloning, DNA Sequencing
• No. of Reactions: 50 Preps