Thermo Scientific™ EZ-Link™ Sulfo NHS-SS Biotinylation Kit

Biotinylate protein amines with this cleavable, mid-length (24.3A), water-soluble, NHS-ester biotinylation reagent containing a cleavable disulfide bond in the spacer arm.

Supplier:  Thermo Scientific™ 21445

Catalog No. PI21445

Description

Thermo Scientific™ EZ-Link Sulfo-NHS-SS-Biotin enables simple and efficient biotin labeling of antibodies, proteins and any other primary amine-containing biomolecules in solution.

Highlights:

• Cleavable analog of Sulfo-NHS-LC-Biotin
• Can remove biotin label by cleaving spacer disulfide bond with reducing agents, such as DTT
• Ideal for affinity purification experiments that require efficient elution from immobilized avidin supports (cleaving disulfide bond releases proteins)
• Long spacer arm reduces steric hindrance for binding interactions

Specifications

• Description: EZ-Link Sulfo-NHS-SS-Biotinylation Kit
• Quantity: 10 reactions
• Labeling Scale: 1 to 10 mg
• Solubility: Water
• Labeling Target: Antibodies, Proteins

• Product Type: Sulfo-NHS-SS Biotinylation Kit
• Content And Storage: Sufficient For: 10 labeling reactions, each with 1 to 10 mg of antibody
  • EZ-Link Sulfo-NHS-SS-Biotin, 25 mg
  • PBS Pack (makes 500 mL), 1 pack
  • Zeba Spin Desalting Columns (7K MWCO), 5 mL, 10 columns
  • HABA Solution, 1 mL
  • Avidin, 10 mg
  
  Store avidin at -20°C. Store biotin reagent desiccated at 4°C. Store remaining kit components at 4°C.
• Product Line: EZ-Link™
• Label or Dye: Biotin

For Research Use Only. Not for use in diagnostic procedures.