Gibco™ ExpiFectamine™ Sf Transfection Reagent

Gibco™ ExpiSf™ Transfection Reagant is a cationic lipid-based reagent for efficient DNA transfection of insect cells using fast, flexible protocols.

Supplier:  Gibco™ A38915

Catalog No. A38915

Description

• Delivers robust and reproducible transfection, giving you greater confidence in your results
• Enables transfection of plasmid DNA in adherent and suspension Sf9 and Sf21 cell cultures for reliable recombinant baculovirus production
• Facilitates efficient production of high-titer, high-quality P0 recombinant baculovirus in suspension culture format without the need for further virus amplification
• Exhibits low toxicity and does not require medium change post-transfection
• Is part of a simple, fast protocol with a short 5-minutes complexation time
• Demonstrates linear scalability of transfections from culture volumes of 4mL to 500mL in suspension format

The ExpiFectamine Sf Transfection Reagent is a core component of the ExpiSf Expression System and has been optimized for transfection of ExpiSf9™ cells (Cat. No. A35243). However, the reagent also demonstrates superior performance using other Sf9 and Sf21 insect cells cultured in serum-free media such as Sf-900 II SFM or Sf-900 III SFM in both adherent and suspension culture formats.

Specifications

• Content And Storage: Store at 2–8°C, do not freeze
  
  Shelf Life: 24 months from date of manufacture
  
  
• Sample Type: Baculovirus DNA, Plasmid DNA
• For Use With (Application): Transfection
• Product Line: ExpiFectamine™
• Quantity: 1 mL
• Shipping Condition: Approved for shipment at Room Temperature or on Wet Ice

• Cell Type: Insect Cells
• Transfection Technique: Lipid-based Transfection
• High-throughput Compatibility: High-throughput Compatible
• Product Type: Transfection Reagent
• Serum Compatible: Yes