Gibco™ ExpiFectamine™ 293 Transfection Kit

Designed for transient transfection of high density cultures of HEK (human embryonic kidney) 293 cells.

Supplier:  Gibco™ A14524

Catalog No. A14524

Description

• The ExpiFectamine™ 293 Transfection Kit is a core component of the Expi293™ Expression System
• It is designed for transient transfection of high density cultures of HEK (human embryonic kidney) 293 cells
• The high-efficiency, cationic, lipid-based transfection reagent and transfection enhancers are designed to power the highest possible level of protein expression from Expi293F™ cells cultured in Expi293™ Expression Medium
• Achieves protein yields 2- to 10-fold higher than other transfection reagents used on high density 293 cell cultures
• Employs the same transient expression protocols typically used in current low-density 293 suspension culture systems to easily switch from low density systems to the high yield, high density Expi293™ Expression System
• Provides robust and reproducible transfection results, giving users greater confidence in their results
• Scale transfections for culture volumes of less than 1 mL to greater than 10 liters, while maintaining equivalent volumetric protein yields
• Store at 2°C to 8°C

Specifications

• Content And Storage: 2.7 mL (3 x 0.9 mL) ExpiFectamine™ 293 Reagent
  5 mL ExpiFectamine™ 293 Transfection Enhancer 1
  50 mL ExpiFectamine™ 293 Transfection Enhancer 2
  
  Sufficient for transfection of 1 L of culture
  
  Store at 2°C to 8°C
• Product Type: Transfection Kit
• Classification: Animal Origin-Free, Chemically Defined, Protein-Free, Serum-Free
• Product Line: ExpiFectamine™
• Serum Compatible: No
• Sample Type: Plasmid DNA

• Quantity: For 1 L of culture
• Cell Line: Expi293F™
• For Use With (Application): Transfection
• Purity: Cell Culture Grade
• Cell Type: Established Cell Lines
• Transfection Technique: Lipid-Based Transfection

For Research Use Only. Not for use in diagnostic procedures.