Gibco™ Expi293™ Expression Medium

Expi293™ Expression Medium

Supplier:  Gibco™ A1435102

Catalog No. A1435102

Description

Expi293™ Expression Medium is a chemically defined, serum-free, protein-free medium for growth and transfection of suspension-adapted human embryonic kidney (HEK) 293 cells. It is specifically designed as a core component to support high density culture of Expi293F™ cells in the Expi293™ Expression System for scalable transient protein expression.

 

• Supports growth of suspension 293 cultures to densities of over 1.5 x 10^7 cells/mL
• Transfection compatible serum-free suspension culture medium enables transfection efficiencies of greater than 70% using ExpiFectamine™ transfection reagent
• Enables sustained, high level expression of high density transiently transfected cultures, achieving yields of up to 1 gram per liter of recombinant protein
• Supports growth and transfection of 293 cells in culture formats of less than 1 mL in multiwell plates to greater than 10 L in disposable bioreactors
• Does not contain phenol red

Expi293™ Expression Medium is formulated with GlutaMAX™ -I reagent. It is ready to use, with no supplementation required. The chemically defined formulation results in high reproducibility and lot-to-lot reliability. Expi293™ Expression Medium contains no human or animal-origin components. The medium is not recommended for adherent cell cultures.

Specifications

• Content And Storage: Store in the dark at +2°C to +8°C
• Cell Line: 293E (HEK), 293 (HEK), Expi293F™, FreeStyle™ 293-F Cells, 293F
• Classification: Animal Origin-free, Chemically-defined, Protein-free, Serum-free
• Culture Type: Suspension Cell Culture
• Product Type: Expression Medium
• Sterility: Sterile
• Without Additives: No HEPES, No Phenol Red, No Hypoxanthine, No Thymidine, No Insulin, No Vitamin A, No 2-Mercaptoethanol
• Format: Bottle(s)
• Inorganic Salts: Calcium, Magnesium, Sodium Phosphate
• Product Line: Expi293™
• Quantity: 6 x 1 L

• Culture Environment: CO₂
• Cell Type: Embryonic Kidney Cells
• Concentration: 1 X
• Form: Liquid
• Serum Level: Serum-free
• With Additives: GlutaMAX, Sodium Pyruvate, Sodium Bicarbonate, Dextrose, Pyridoxine HCl
• Endotoxin Level: < 1 EU/ml
• Included Antibiotics: No antibiotics
• Osmolality: 275-295 mOsm/kg
• Purity or Quality Grade: Cell Culture Grade

For Research use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.