Abnova™ ERG Split CISH Probe

ERG Split CISH Probe is designed for the qualitative detection of human ERG gene at 21q22.2 in formalin-fixed, paraffin-embedded specimens by chromogenic in situ hybridization (CISH).

Supplier:  Abnova™ CS0007

Catalog No. 89-335-542

Description

The probe is intended to be used in combination with the CISH Implementation Kit 2, which provides necessary reagents for specimen pretreatment and post-hybridization processing.

Hybridization signals of digoxigenin-labeled polynucleotides appear dark green distinct dot-shaped (distal to the ERG breakpoint region), and dinitrophenyl-labeled polynucleotides appear bright red distinct dot-shaped (proximal to the ERG breakpoint region).
Normal situation: In interphases of normal cells or cells without a translocation involving the ERG gene region, two red/green fusion signals appear.
Aberrant situation: In a cell with deletions affecting the ERG gene region resulting in the TMPRSS2-ERG fusion, the loss of one green signal will be observed. One ERG gene region affected by a translocation without involvement of TMPRSS2 (e.g, SLC45A3-ERG) is indicated by one separate green signal and one separate red signal. Genomic aberrations due to small deletions, duplications or inversions might result in inconspicuous signal patterns. Other signal distribution may be observed in some abnormal samples which might result in a different signal pattern than described above, indicating variant rearrangements.
Unexpected signal patterns should be further investigated.

Specifications

• Content And Storage: The product is ready-to-use. No reconstitution, mixing, or dilution is required. Bring probe to room temperature (18 to 25°C) and mix briefly before use.
• For Use With (Application): CISH-P