Invitrogen™ Equine IL-4, Recombinant Protein

Recombinant Protein

Supplier:  Invitrogen™ REIL4I

Catalog No. ENREIL4I

Description

Equine IL-4 Recombinant Protein, REIL4I, from Invitrogen.

Interleukin-4 (IL-4) is a pleiotropic, immune-modulatory cytokine produced by activated T cells, and serves as a ligand for interleukin 4 receptor. IL-4 has been found to have the broadest range of actions. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play an important role in mediating the immune regulatory signal of IL-4. The IL-4 gene, IL-3, IL-5, IL-13, and CSF2 form a cytokine gene cluster on chromosome 5q. In particular, IL-4 is located in close proximity to IL13 on the chromosome. Further, the IL-4 gene, IL13 and IL5 are found to be coordinated by several long-range regulatory elements over a 120 kilobase range on the chromosome. IL-4 delta 2 is a known splice variant of IL-4. Reconstitute with 0.5 mL of distilled or deionized water. For dilution, use a buffer containing carrier protein, e.g. PBS with 1% BSA or a neutral media containing 10% FCS and freeze in aliquots at -70°C. The biological activity of recombinant equine IL-4 was determined by measuring the proliferation of TF-1 cells. The ED50 (or 1 unit of activity) for this effect in our laboratories typically falls into the range of 20 - 50ng/mL. Ref: Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. Mature equine IL-4 was expressed in Pichia pastoris, X-33. GenBank™ Accession &Num; P42202, amino acids 24 - 132. For protein verification, the expressed protein was N-terminal sequenced and begins with the following 10 amino acids: CKYDITLQEI. Reconstitute with 0.5 mL of distilled water.

Specifications

• Accession Number: P42202
• Formulation: PBS with no preservative
• Name: Equine IL-4
• Source: Yeast
• Regulatory Status: RUO
• Common Name: Equine IL-4
• Specific Reactivity: Equine
• Cross Reactivity: Eq
• Recombinant: Recombinant
• Protein Subtype: Recombinant

• For Use With (Application): ELISA, Immunoprecipitation, Western Blot
• Gene ID (Entrez): 100034225
• Quantity: 20 μg
• Storage Requirements: 4°C
• Gene Alias: Equine Interleukin 4 IL4
• Gene Symbol: IL4
• Conjugate: Unconjugated
• Species: Yeast
• Form: Lyophilized
• Purity or Quality Grade: purified

Safety and Handling

ShelfLife : 12 Months