Abnova™ EML4 Split CISH Probe

EML4 Split CISH Probe is designed for the qualitative detection of human EML4 gene at 2p21 in formalin-fixed, paraffin-embedded specimens by chromogenic in situ hybridization (CISH).

Supplier:  Abnova™ CS0006

Catalog No. 89-335-540

Description

The probe is intended to be used in combination with the CISH Implementation Kit 2, which provides necessary reagents for specimen pretreatment and post-hybridization processing.

Hybridization signals of digoxigenin-labeled polynucleotides appear dark green distinct dot-shaped (distal to the EML4 breakpoint region), and dinitrophenyl-labeled polynucleotides appear bright red distinct dot-shaped (proximal to the EML4 breakpoint region).
Normal situation: In interphases of normal cells or cells without a translocation involving the EML4 gene region, two red/green fusion signals appear.
Aberrant situation: One EML4 gene region affected by a translocation is indicated by one separate green signal and one separate red signal. Genomic aberrations due to small deletions, duplications or inversions might result in inconspicuous signal patterns. Other signal distribution may be observed in some abnormal samples which might result in a different signal pattern than described above, indicating variant rearrangements.
Unexpected signal patterns should be further investigated.

Specifications

• For Use With (Application): CISH-Ce
• Content And Storage: The product is ready-to-use. No reconstitution, mixing, or dilution is required. Bring probe to room temperature (18 to 25°C) and mix briefly before use.