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MilliporeSigma™ Introductory pETBlue-2 Perfectly Blunt Cloning Kit
Supplier: MilliporeSigma™ 706353
Description
The Perfectly Blunt™ Cloning Kits are designed for simplified cloning of DNA generated by PCR using any type of DNA polymerase. This approach enables the use of high-fidelity proofreading enzymes for amplification, thus decreasing the probability of generating mutations in the target sequence. In addition, under many conditions blunt cloning is more efficient than T-cloning, most likely due to the observation that the efficiency of single dA addition by Taq DNA polymerase varies significantly depending on the sequence context of the DNA ends, and even the number of PCR cycles performed.Specifications
| Cloning of DNA generated by PCR; Cloning restriction fragments, cDNA, or sheared DNA | |
| Store at -70°C. | |
| 10 rxn |
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