MilliporeSigma™ Upstate™ Histone H3 (Unmodified Lys4)α, Mouse monoclonal, ChIP Validated Antibody and Primer Set

Mouse Monoclonal Antibody

Supplier:  MilliporeSigma™ 17675

Catalog No. 17-675-MI

Description

Specifically detects Histone H3 (Unmodified Lys4)α Clone: in Vertebrate samples, and it is validated for Chromatin Immunoprecipitation, Western Blotting

Specifications

• Antigen: Histone H3 (Unmodified Lys4)α
• Conjugate: Unconjugated
• Gene Accession No.: Q66I33
• Gene Symbols: HIST3H3; H3FT; H3/t; H3t; H3/g; H3.4; H3T
• Immunogen: The histone H3 (Unmodified Lys4) purified antibody is made against a synthetic peptide corresponding to amino acids 1-12 of histone H3.
• Purification Method: Protein G purified
• Regulatory Status: RUO
• Storage Requirements: −20°C for one year from date of shipment; Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution; Aliquot into microcentrifuge tubes and store at −20°C; Avoid repeated freeze/thaw cycles
• Monoclonal or Polyclonal: Monoclonal
• Test Specificity: Recognizes unmodified histone H3 (Lys4), Mr 17kDa. Epitope specificity of antibody as determined by ELISA maps near Lys4. Antibody tolerates modifications on Lys9 and Ser10 but not Lys4 or Thr31.
• Target Species: Vertebrates

• Applications: ChIP assay, Western Blot
• Formulation: Anti-Histone H3 (Unmodified Lys4) (mouse monoclonal IgG2bκ, clone CMA301). One vial containing 50μg of protein G purified antibody in 50μL PBS containing 0.05% sodium azide. Normal Mouse IgG. Two vials containing 25μg purified Mouse IgG in 25μL storage buffer containing 0.1% sodium azide. ChIP Primers β-Globin. One vial containing 75μL of 5μM of each primer specific for the human β-globin promoter. FOR: AGG ACA GGT ACG GCT GTC ATC; REV: TTT ATG CCC AGC CCT GGC TC
• Format: Purified
• Host Species: Mouse
• Isotype: IgG2b, κ
• Quantity: 25 Assays
• Research Discipline: Epigenetics and Nuclear Function
• Primary or Secondary: Primary
• Gene ID (Entrez): NM_003493
• Includes: This ChIPAb+ Histone H3 (Unmodified Lys4) -ChIP Validated Antibody and Primer Set conveniently includes the antibody and the specific control PCR primers.

For Research Use Only