MilliporeSigma™ Upstate™ Androgen Receptor- ChIP Validated, Rabbbit Polyclonal, ChIP Validated Antibody and Primer Set

Rabbit Polyclonal Antibody

Supplier:  MilliporeSigma™ 1710489

Catalog No. 17-104-89MI

Description

Specifically detects Androgen Receptor- ChIP Validated Clone: in Amphibian, Bovine, Chicken, Human, Mouse, Rat samples, and it is validated for Chromatin Immunoprecipitation, Immunohistochemistry, Immunoprecipitation, Western Blotting

The androgen receptor (AR) is a type of nuclear receptor which is activated by binding of either of the androgenic hormones testosterone or dihydrotestosterone. The main function of the androgen receptor is as a DNA binding transcription factor which regulates gene expression. However the androgen receptor also has additional functions independent of DNA binding. The androgen receptor is most closely related to the progesterone receptor, and progestins in higher dosages can block the androgen receptor.

Specifications

• Antigen: Androgen Receptor- ChIP Validated
• Conjugate: Unconjugated
• Gene Accession No.: P10275
• Gene Symbols: AR, DHTR, SMAX1, HUMARA, KD, SBMA, TFM, OTTHUMP00000061928, AIS, NR3C4
• Immunogen: Peptide corresponding to amino acids 1-21 of the Human Androgen Receptor (MEVQLGLGRVYPRPPSKTYRG)
• Quantity: 25 Assays
• Research Discipline: Epigenetics and Nuclear Function
• Primary or Secondary: Primary
• Gene ID (Entrez): NP_000035
• Includes: ChIPAb+ Androgen Receptor set includes the Androgen Receptor antibody, a Normal Rabbit IgG, and control primers, which amplify a 85bp region of the human prostate specific antigen (PSA) enhancer region.

• Applications: ChIP assay, Immunohistochemistry, Immunoprecipitation, Western Blot
• Formulation: Anti-Androgen Receptor (Rabbit Polyclonal). One vial containing 125μL (0.2μg/μL) purified rabbit polyclonal in buffer containing 0.1M Tris-Glycine (pH 7.4), 150mM NaCl, and 0.05% sodium azide, before the addition of 30% glycerol. Normal Rabbit IgG. One vial containing 125μg (1.0μg/μL) Rabbit IgG in 125μL storage buffer containing 0.05% sodium azide. ChIP Primers, PSA. One vial containing 75μL of 5μM of each primer specific for human prostate-specific antigen enhancer region. (chr19:51354179+51354263, hg19 build) FOR: 5’ GCC TGG ATC TGA GAG AGA TAT CAT C 3’; REV: 5’ ACA CCT TTT TTT TTC TGG ATT GTT G 3’
• Format: Affinity Purified
• Host Species: Rabbit
• Purification Method: Immunoaffinity purified
• Regulatory Status: RUO
• Storage Requirements: −20°C for up to 12 months from date of receipt; Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution; Aliquot into microcentrifuge tubes and store at −20°C; Avoid repeated freeze/thaw cycles
• Monoclonal or Polyclonal: Polyclonal
• Test Specificity: Recognizes Androgen Receptor. No cross-reactivity with estrogen, progesterone or glucocorticoid receptors.
• Target Species: Amphibian, Bovine, Chicken, Human, Mouse, Rat

For Research Use Only