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Edvotek™ Identification of Bacterial Protein Profiles
Compare total protein extracts from several bacterial sources.
Supplier: Edvotek™ 252
Includes:
- Instructions
- Bacterial cultures and reagents
- LyphoProteins™
- Lysozyme, buffers
- Protein InstaStain™
- Practice gel loading solution
- Protein sample buffer
- Unknown proteins ready for electrophoresis
- ReadyPour™ Agar
- Nutrient broth
Description
- The unique patterns of protein bands, obtained by SDS vertical polyacrylamide electrophoresis, can be used to identify various bacterial strains
Experiment times:
- Day one setup, 15 minutes
- Day two setup, one hour
- Electrophoresis, one hour
- Staining, 20 minutes
- Destaining, two hours
All You Need:
- 3 polyacrylamide gels (12%)
- Vertical gel electrophoresis apparatus
- Power supply
- Microcentrifuge
- Incubation oven
- Hot plate or burner
- White light visualization system
- Automatic micropipet with fine tips
- Lab glassware
- Methanol
- Glacial acetic acid
- Distilled water.
Order Info
For six groups sharing three gels.
Recommended Storage
Some components require freezer storage
Specifications
| Fingerprinting of Bacterial Proteins | |
| Compare total protein extracts from several bacterial sources | |
| 6 Lab Groups |
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