Edvotek™ Advanced Placement Laboratory Kit 2: Principles of Enzyme Catalysis

Understand enzyme catalysis and protein structure-function relationships.

Supplier:  Edvotek™ 282

Catalog No. S11282

Includes:

• Catalase solution
• 1.2% Hydrogen peroxide (stabilized)
• Phosphate buffer, pH 7.2 (20x concentrate)
• Assay reagent (potassium iodide concentrate)
• Acidification solution, 0.1M HCl
• Color enhancer (concentrate)
• Color developer (concentrate)
• Plastic tube, 15mL
• Comprehensive instructions, background material, and study questions

Description

Methodology uses a colorimetric assay to measure the concentration of hydrogen peroxide (and therefore catalase activity) based on the oxidation of iodide by peroxide. This method differs from the AP Biology lab manual; it uses a safer system that eliminates the need for sulfuric acid.

• Perform an enzyme assay
• Determine the rate of a biochemical reaction

Specifications

• Product Type: AP Biology Lab Kit 2: Principles of Enzyme Catalysis
• Includes: Catalase solution, 1.2% Hydrogen Peroxide, 20X pH 7.2 Phosphate Buffer, Potassium Iodide concentrate assay reagent, 0.1M HCl acidification solution, color enhancer, color developer, 15mL Plastic tube, comprehensive instructions, background material and study questions
• Content And Storage: Refrigerated

• For Use With (Application): Understand enzyme catalysis and protein structure function relationships
• Time Range: 30 to 45 min.
• Class Size: 40 Students