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Thermo Scientific™ DNAPac™ RP Columns
Get high resolution LC and LC-MS separation of oligonucleotides, including double-stranded (ds) DNA/RNA fragments, using these DNAPac reversed phase columns.
Supplier: Thermo Scientific™ 0809221521232
Description
Achieve superior reversed phase oligonucleotide separations using the Thermo Scientific™ DNAPac™ RP HPLC columns. The unique chemistry is designed for analysis of oligonucleotides and double-stranded (ds) DNA/RNA fragments using LC-UV or LC-MS. The column chemistry provides excellent performance under a broad range of pH, temperature, and mobile phase compositions. In addition, the wide pore size of the resin provides excellent separation of large double-stranded nucleic acids up to 10k base pairs.
DNAPac RP reversed phase (RP) column is based on spherical wide-pore 4 μm polymer resin specifically designed for separation of oligonucleotides and DNA/RNA fragments. The stationary phase is highly stable to extreme pH (0-14), and temperature (5-110°C) and fully compatible with mass spectrometry friendly organic solvents and ion-pair reagents such as triethylamine (TEA) and hexylamine (HA). The wide pore size of polymer resin enables efficient separation of small to large nucleic acids with low carry-over. The column may be operated in both HPLC and UHPLC instruments, producing high resolution at high throughput for oligonucleotides and resolves both small and very large dsDNA segments:
- Designed for ion-pair reversed-phase (IP-RP) LC and LC/MS separations of both oligonucleotides and double-stranded nucleic acids
- High resolution and high throughput
- Excellent MS compatibility
- Wide operating pH range (0-14) and high temperature stability (up to 110°C)
- Biocompatible hardware to eliminate analyte absorption
Specifications
| Preparative column, standard | |
| 4 to 8 mL/min | |
| 2900 psi (200 bar) | |
| 0 to 14 | |
| up to 110°C | |
| DNAPac | |
| Reversed Phase | |
| DVB (Divinylbenzene) Polymer | |
| Reversed Phase HPLC |
| 21.2 mm | |
| 150 mm | |
| 4 μm | |
| Each | |
| Proprietary | |
| 4 µm polymer Resin | |
| Reversed Phase | |
| Preparative Column |
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