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Thermo Scientific™ DNA Polymerase I (10 U/μL)

DNA Polymerase I is a template-dependent DNA polymerase which catalyzes 5'→3' synthesis of DNA.

Supplier:  Thermo Scientific™ EP0042

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Catalog No. FEREP0042


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Description

Description

DNA Polymerase I, a template-dependent DNA polymerase, catalyzes 5'→3' synthesis of DNA. The enzyme also exhibits 3'→5' exonuclease (proofreading) activity, 5'→3' exonuclease activity, and ribonuclease H activity.

Highlights

  • Incorporates modified nucleotides(e.g. biotin-, digoxigenin-, aminoallyl-, fluorescently-labeled nucleotides)
  • Active in multiple buffers, including restriction enzyme, PCR, and RT buffers

Applications

  • DNA labeling by nick-translation in conjunction with DNase
  • Second-strand synthesis of cDNA in conjunction with RNase
Specifications

Specifications

DNA Polymerase I
DNA Polymerase I
2500 U
10 U/μL
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For Research Use Only. Not for use in diagnostic procedures.