Gibco™ DMEM, high glucose, GlutaMAX™ Supplement

Used as basal medium for supporting the growth of many different mammalian cells. Gibco™ DMEM, High Glucose, GlutaMAX™ Supplement is used in human ex vivo tissue and cell culture processing applications.

Catalog Number	Mfr. No.	Quantity
10-566-016	Gibco™ 10566016	500 mL
10-566-024	Gibco™ 10566024	10 x 500 mL
10-566-032	Gibco™ 10566032	5 L

Description

• Contains 4 times the concentration of amino acids and vitamins than the original Eagle's Minimal Essential Medium
• GlutaMAX supplement minimizes toxic ammonia build-up and improves cell viability and growth in easy-to-use format
• Originally formulated with low glucose (1g/L) and sodium pyruvate, but is often used with higher glucose levels, with or without sodium pyruvate
• Contains no proteins, lipids, or growth factors
• Requires supplementation, commonly with 10% Fetal Bovine Serum (FBS)
• Uses sodium bicarbonate buffer system (3.7g/L), and therefore requires a 5 to 10% CO₂ environment to maintain physiological pH
• Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, cell lines such as HeLa, 293, Cos-7, and PC-12

Order Info

Shipping Condition: Room Temperature

Specifications

• With Additives: High Glucose, GlutaMAX, Phenol Red
• Without Additives: No HEPES, No Sodium Pyruvate

Safety and Handling

ShelfLife : 12 Months From Date of Manufacture

When used as a medical device, Federal law restricts this device to sale by or on the order of a physician. Customers using Gibco DMEM in a manufacturing process, who have a submission with the FDA, may request a letter of authorization from Life Technologies to reference their Type II Drug Master File. Class II Device U.S. For human ex vivo tissue and cell culture processing applications.