Thermo Scientific™ pCMV-Cypridina Luc Vector for Luciferase Assays

Drive Cypridina luciferase expression as a reporter for gene regulation studies using vectors with a multiple cloning site (MCS) or control promoter (CMV or TK).

Supplier:  Thermo Scientific™ 16150

Catalog No. PI16150

Description

The Thermo Scientific™ pMCS-Cypridina Luc Vector is a multiple cloning site plasmid designed to accept a promoter sequence for study of gene regulation using the naturally secreting Cypridina luciferase reporter.

The Cypridina Luc Vectors contain a gene cloned from the marine ostracod, Cypridina noctiluca. The gene encodes a naturally-secreted bioluminescent Cypridina luciferase (62kDa), which enables measurement of the reporter activity in media (for real-time assays) and in cell lysates. The pMCS vector contains a multiple cloning site for cloning a promoter to study its regulatory potential. The pCMV and pTK vectors have the luciferase gene under the CMV (Cytomegalovirus) promoter and Herpes Simplex Virus (HSV) thymidine kinase (TK) promoter, respectively. These constitutive expression vectors can be used as normalization controls to account for experimental variation in combination with other reporters.

Highlights:

• Naturally-secreting Cypridina luciferase gene, optimized for high expression in mammalian systems
• Multiple cloning site (MCS) provides versatility for transfer of regulatory elements from one plasmid to another
• Transcription termination site (Ter), Lac operator (Lac O1), and transcriptional pause site (TPS) used to minimize background by reducing transcriptional read-through
• Both puromycin (Pur) and ampicillin (Amp) markers for drug selection in mammalian and bacterial cells, respectively
• High-copy pUC bacterial DNA replication origin
• Two control vectors available with strong (CMV) or weak (TK) constitutive promoters for co-transfection and normalization

Specifications

• Description: pCMV-Cypridina Luc Vector
• Cloning Method: Restriction Enzyme/MCS
• Quantity: 10 μg
• Reporter Gene: Cypridina Luciferase
• Product Line: Pierce™
• Promoter: CMV

• Type: Luc Vector
• Delivery Type: Transfection
• Content And Storage: Store between -70°C and -20°C.
• Selection Agent (Eukaryotic): Puromycin
• For Use With (Application): Reporter Assays

For Research Use Only. Not for use in diagnostic procedures.