Invitrogen™ CSPD™ Substrate (0.25 mM Ready-To-Use) with Nitro-Block™ Enhancer

Ready-to-use chemiluminescent alkaline phosphatase substrate, for protein or nucleic acid blotting on nitrocellulose membranes or solution-based assays.

Supplier:  Invitrogen™ T2217

Catalog No. T2217

Description

Chemiluminescent substrates provide highly sensitive replacements for the widely used fluorogenic substrate methylumbelliferyl phosphate (MUP), and the colorimetric substrate p-nitrophenyl phosphate (pNPP). Low background luminescence coupled with high-intensity light output enables detection of alkaline phosphatase labels with the highest possible sensitivity and signal-to-noise.

• Detect alkaline phosphatase and alkaline phosphatase-labeled molecules with unparalleled sensitivity, speed and ease
• Exhibits high sensitivity in membrane-based applications such as Southern, Northern, and Western blotting
• Use in solution-based assays such as immunoassays, DNA probe assays, enzyme assays, and reporter gene assays
• Maximum light levels are reached in approximately 10 minutes and glow emission persists for several hours
• Enhancers increase light output and sensitivity

Specifications

• Product Type: ELISA Substrate
• Form: Liquid
• Product Line: CSPD™, Nitro-Block™, NovaBright™
• Content And Storage: Store the product at 4°C.

• Detection Method: Chemiluminescence
• Format: Bottle
• Substrate Type: Alkaline Phosphatase
• Shipping Condition: Room Temperature

For Research Use Only. Not for use in diagnostic procedures.