Invitrogen™ Countess™ 3 Automated Cell Counter

Experience rapid assessment of cells with the Countess 3 FL Automated Cell Counter. Equipped with advanced optics, automated focus and lighting, and AI-based image analysis software, the Countess 3 FL enables counting cells and isolated nuclei, monitoring fluorescence, and measuring cell viability.

Supplier:  Invitrogen™ A50302/PKG

Catalog No. A50302

Description

The Countess 3 FL Automated Cell Counter is a benchtop assay platform equipped with state-of-the-art optics, fully automated focus and lighting, and image analysis software driven by a neural network-based machine-learning algorithm for rapid automated cell counting of primary and immortalized cell lines. The Countess 3 FL counter is capable of brightfield or fluorescence illumination with three-channel flexibility (brightfield and two optional fluorescence channels) enabling researchers to count cells and isolated nuclei, monitor fluorescence, examine apoptosis, and measure cell viability.

Features of the Countess 3 Automated Cell Counter include:

• Accuracy—highly accurate machine-learning algorithm applied to automated cell counting, eliminates the subjectivity of manual cell counting and user-to-user variability
• Time savings—counts live and dead cells, measures viability, calculates percent cell aggregates, and reports average cell size with a single touch in about 20 seconds
• Convenience—requires no cleaning, routine maintenance, or calibration; flush-mounted high-resolution capacitive touchscreen and simple user interface provide quick startup, require minimal training, and offer automatic saving of data to USB or cloud
• Flexibility—compatible with both disposable and reusable cell counting chamber slides

The Countess 3 Automated Cell Counter works in two simple steps:

• Mix 10 μL of sample with 10 μL of trypan blue and pipet into a disposable or reusable Countess chamber slide
• Insert the slide into the instrument. Rapid Capture and Auto-Save modes allow automated cell counting with a single touch. Focus, lighting, and file saving are executed automatically

Obtain accurate cell counts

The Countess 3 Automated Cell Counter uses trypan blue staining combined with auto-focus and lighting driven by a machine-learning image analysis algorithm to obtain accurate cell and viability counts. The measurement range extends from 1 x 10⁴ to 1 x 10⁷ cells/mL (the optimal range is 1 x 10⁵ to 4 x 10⁶ cells/mL), which is broader than that of a hemocytometer

Transfer data and images to your PC or cloud

Using the USB port on the Countess 3 Automated Cell Counter and any USB drive, you can save your cell count results and images and transfer them to your PC. For your convenience, a USB drive is supplied with the Countess 3 instrument, and a WiFi dongle is available.

Improve downstream single-cell analysis

Minimizing cell aggregates is crucial for RNA sequencing applications to ensure that individual cells are partitioned and barcoded during the single-cell library preparation process. For optimal single-cell data output, the percent cell aggregates on the Countess 3 Automated Cell Counter can be used to confirm that the starting sample has <5% aggregates before performing downstream single-cell analysis.

The Countess 3 Automated Cell Counter can be purchased as the instrument (Cat. No. AMQAX2000) or a variety of packages bundled with additional accessories and extended warranties:

• Countess 3 Starter Package (Cat. No. A49865) which includes:
  • Countess 3 Automated Cell Counter
  • Reusable slide
  • 500 disposable slides with trypan blue

Extended warranty instrument and starter packages

The Countess 3 Automated Cell Counter comes with a standard 1-year warranty. Keep your cell counter up and running with the addition of a 3-year extended warranty that covers all costs for shipping, parts, and labor for repairs. Countess 3 instrument and Starter Packages are available with two different types of extended warranty packages:

• Rapid Exchange (REX) Extended Warranty (3 years)—replacement shipped out in 1 business day for labs with critical timelines
• Available with the instrument only (Cat. No. A49862) and the Countess 3 Starter Package (Cat. No. A50298)
• AB Repair Center (ABRC) Extended Warranty (3 years)—instrument repaired and returned in ~3 weeks for regulated labs required to maintain asset tagging consistency (loaner instrument shipped within 1 business day with upgrade to an ABRC Support Plus contract)
• Available with the instrument only (Cat. No. A49891) and the Countess 3 Starter Package (Cat. No. A50302)

21 CFR Part 11 Software Module

The Countess 3 21 CFR Part 11 Software Module is for use with the Countess 3 and Countess 3 FL automated cell counters and supports compliance with 21 CFR Part 11 FDA guidelines for security, auditing, and electronic (SAE) signatures using an SAE administration console. The 21 CFR Part 11 software can be purchased separately (Cat. No. A51025).

Specifications

• Type: Automated Cell Counter Starter Package
• Camera: 5 MP
• For Use With (Equipment): EVOS Microscopes, Countess Chamber Slides, and Reusable Slide
• Light Source: Brightfield
• Optics: 1 Channel (Brightfield)
• Memory: Onboard, USB drive
• Weight (English): 8 lb.
• Certifications/Compliance: RoHS
• Shipping Condition: Ambient
• Electrical Requirements: 12 VDC, 3 A

• Product Line: Countess
• For Use With (Application): Cell Counting and Viability Measurement
• Detection Method: Brightfield
• Magnification: 2.5X
• Display: LCD (1280 x 800 pixels)
• Dimensions: 9 x 9 x 5.5 in. (228.6 x 228.6 x 139.7 mm)
• Weight (Metric): 3.6 kg
• Content And Storage:
  • Countess™ 3 Automated Cell Counter, 1 each
  • Power cord with 4 adapters (for US/Canada/Taiwan/Japan, Europe, or UK), 1 each
  • Countess™ Cell Counting Chamber Slides, 50 slides/box
  • Countess™ 3 Disposable Slide Holder, 1 each
  • Countess™ 3 Reusable Slide Holder, 1 each
  • Countess™ 3 USB Drive, 1 each
  • Countess™ 3 Automated Cell Counter Quick Reference Card, 1 each
  • Countess™ Reusable Slide (Cat. No. A25750), 1 each
  • Disposable Countess™ Cell Counting Chamber Slides (Cat. No. C10283), 10 x 50 slides
  • Trypan blue (Cat. No. T10282), 20 x 1 mL vials

  Store at room temperature.

• Warranty: 3-year AB Repair Center (ABRC) Extended Warranty (1 year standard warranty + 2 years extended repair coverage)
• Voltage: 100/240 V

For Research Use Only