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Corning™ HEPES, Liquid
An alternative to use of bicarbonate for buffering
Supplier: Corning™ 25060CI
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Description
Technical Advantage: Product manufactured under industry-leading standards for low endotoxin content and superior results.HEPES (N-2-hydroxyethylpiperazine-N'-2-ethanesulphonic acid) provides an alternative to the use of bicarbonate for buffering because it does not require elevated levels of CO2. Unlike the low pKa of bicarbonate, HEPES has a pKa of 7.66 making it a much stronger buffer in the pH 7.2-7.6 range than bicarbonate buffer. Potential disadvantages of HEPES include toxicity over 100 mM and lack of nutritive value to the cell. The recommended working concentration is 10-25 mM.
- Complete Certificate of Analysis available for each production lot, along with full formulation information
Corning media certificates are available on our Resource Library.
Specifications
| 15° to 30°C | |
| HEPES Buffer | |
| 7.2 to 7.6 |
| Liquid | |
| Sterile-filtered | |
| 6 x 100 mL |
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