Cytiva Con A Sepharose™ 4B Medium

An affinity chromatography media for capture of glycosylated biomolecules including glycoproteins and glycolipids

Supplier:  Cytiva 17044003

Catalog No. 45-000-069

Description

Highly suited for isolation of cell surface glycoproteins from detergent-solubilized membranes.

• Con A coupled to Sepharose, in a 4% agarose matrix via cyanogen bromide activation
• Binds molecules that contain α-D-mannose, α-D-glucose, and sterically related residues with available C-3, C-4, or C-5 hydroxyl groups
• Group-specific adsorbent for molecules containing sugars
• Concentration of immobilized Con A, approx. 13mg/mL medium; binding capacity for porcine thyroglobulin, approx. 30mg/mL medium
• Stable in the pH range 4 to 9, and in the presence of all commonly used buffers
• Average particle size: 90μm
• Delivered in 0.1M acetate buffer, pH 6.0, containing 1M NaCl, 1mM CaCl₂, 1mM MgCl₂, 1mM MnCl₂, and 20% ethanol

Specifications

• Binding Capacity: 30mg/mL
• Matrix: 4% Agarose
• pH: 4 to 9
• Content And Storage: 2°C to 8°C
• Ligand: Concanavalin A

• Ligand Concentration: 13mg Con A/mL Drained Medium
• Particle Size: 90 μm
• Quantity: 5 mL
• For Use With (Application): For separation and purification of Glycoproteins, Polysaccharides and Glycolipids, isolates cell surface Glycoproteins from detergent-solubilized membranes