Invitrogen™ Click-iT™ sDIBO Alkynes for copper-free click chemistry

Experience copper-free 'click chemistry' with Click-IT™ Alexa Fluor™ and sDIBO Alkyne reagents, which are ideal for surface labeling of live cells and minimize damage to enzymes and fluorescent proteins.

Catalog Number	Mfr. No.	dimLabelorDye	dimReactiveMoiety	Molecular Weight (g/mol)
C10407	Invitrogen™ C10407			∼1200
C20020	Invitrogen™ C20020			∼1400
C20021	Invitrogen™ C20021			∼1800
C20022	Invitrogen™ C20022			∼1900
C20023	Invitrogen™ C20023			∼1000
C20025	Invitrogen™ C20025			∼1050

Description

Achieve efficient labeling of azides with Click-It sDIBO Alkynes. Our Click-IT sDIBO alkynes enable the labeling of azido labeled targets without the need for copper catalysis and are available with a variety of Alexa Fluor or biotin labels, as well as amine or thiol reactive groups for creating your own conjugates. Macromolecules that have been azido-modified enzymatically, chemically, or metabolically can now be labeled easily, yielding soluble bioconjugates.

Click-iT sDIBO Alkyne labels react with azides via a copper-free Click chemistry reaction to form a stable triazole linkage with an Alexa Fluor, Biotin, or label of your choice. Copper-free click reactions using our sDIBO alkynes are ideal for surface labeling of live cells; they also minimize damage to fluorescent proteins like GFP or R-PE. For intracellular labeling, the nonspecific binding of DIBO alkynes creates a background signal, and a labeling strategy using picolyl azides is the preferred solution in these instances.

Click chemistry-labeled molecules can be applied to complex biological samples and be detected with unprecedented sensitivity due to extremely low background. Macromolecules that have been azide-modified enzymatically, chemically, or metabolically can be labeled more easily, yielding more soluble bioconjugates with improved biological labeling utility.

Specifications

• Content And Storage: Store at ≤-20°C. Desiccation recommended. Protect material from long-term exposure to light; may be exposed to light for short periods of time
• Labeling Method: Click Chemistry
• Shipping Condition: Dry Ice
• Green Features: Less hazardous