Chicken anti-Rat IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor™ 488, Invitrogen™

Chicken Polyclonal Secondary Antibody

Supplier:  Invitrogen A21470

Catalog No. A21470

Description

Chicken anti-rat antibodies react with IgG heavy chains and all classes of immunoglobulin light chains from rat. Chicken secondary antibodies have gained popularity because they demonstrate a lower level of nonspecific binding. Chicken antibodies lack a classic “Fc” domain and will not bind to protein A or protein G, nor will they bind to mammalian IgG Fc receptors.

Anti-Rat secondary antibodies are affinity-purified antibodies with well-characterized specificity for rat immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

Specifications

• Antigen: Rat IgG (H+L) Cross-Adsorbed
• Classification: Polyclonal
• Conjugate: Alexa Fluor 488
• Host Species: Chicken
• Purification Method: purified
• Regulatory Status: RUO
• Target Species: Rat
• Form: Liquid

• Applications: Immunocytochemistry, Immunohistochemistry
• Concentration: 2 mg/mL
• Formulation: PBS with 5mM sodium azide; pH 7.5
• Immunogen: Gamma Immunoglobins Heavy and Light chains
• Quantity: 1 mg
• Primary or Secondary: Secondary
• Content And Storage: 4° C, store in dark
• Isotype: IgY