CD4 Rat, anti-Mouse, RB780, Clone: GK1.5, BD Horizon™

Rat Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
BDB568695	BD Biosciences 568695	25 μg
BDB568694	BD Biosciences 568694	0.1 mg

Description

The GK1.5 monoclonal antibody specifically binds to the mouse CD4 (L3T4) differentiation antigen. CD4 is expressed on most thymocytes, a subpopulation of mature T lymphocytes (i.e., MHC class II-restricted T cells, including most T helper cells), and a subset of NK-T cells. In addition, CD4 has also been reported to be detectable on pluripotent hematopoietic stem cells, bone marrow myeloid and B-lymphocyte precursors, intrathymic lymphoid precursors, and a subset of splenic dendritic cells. CD4 has also been reported to be expressed on the plasma membrane of mouse egg cells and is involved in adhesion of the egg to MHC class II-bearing sperm. CD4 is an antigen coreceptor on the T-cell surface which interacts with MHC class II molecules on antigen-presenting cells. It participates in T-cell activation through its association with the T-cell receptor complex and protein tyrosine kinase lck. The GK1.5 antibody reportedly blocks binding of the RM4-5 and H129.19, but not RM4-4 mouse CD4-specific antibodies.

Specifications

• Antigen: CD4
• Concentration: 0.2 mg/mL
• Conjugate: RB780
• Regulatory Status: RUO
• Formulation: Aqueous buffered solution containing ≤0.09% sodium azide
• Immunogen: Mouse CTL clone V4
• Primary or Secondary: Secondary

• Clone: GK1.5
• Applications: Flow Cytometry
• Host Species: Rat
• Target Species: Mouse
• Gene ID (Entrez): 12504
• Isotype: IgG2b κ
• Content And Storage: Refrigerate (2°C to 8°C)