CD271 (NGF Receptor) Monoclonal Antibody (ME20.4), eBioscience™, Invitrogen™

Mouse Monoclonal Antibody

Supplier:  Invitrogen 14940082

Catalog No. 50-112-2782

Description

Description: The monoclonal antibody ME20.4 recognizes CD271, a 75 kDa type I transmembrane protein that belongs to the low-affinity neurotrophin receptor and tumor necrosis factor receptor superfamily. CD271 is expressed by neurons, Schwann cells, melanocytes, mesenchymal stem/stromal cells, neural crest stem cells, astrocytes, oligodendrocytes, and a subset of lymphoid cells including follicular dendritic cells. CD271 binds to the ligands NGF, BDNF, NTF3, and NTF4. CD271 contains a death domain which induces apoptosis and regulates cell growth and proliferation through interactions with TRAF2, TRAF4, TRAF6, PTPN13, and RANBP9. The ME20.4 monoclonal antibody recognizes human, non-human primate, mouse, rat, sheep, rabbit, cat, dog, and pig CD271. Applications Reported: This ME20.4 antibody has been reported for use in flow cytometric analysis, western blotting, microscopy, immunohistochemical staining, and immunocytochemistry. Applications Tested: This ME20.4 antibody has been tested by flow cytometric analysis of SK-N-SH cells. This can be used at less than or equal to 0.25 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. This ME20.

Neurotrophic factors control the survival, differentiation and maintenance of neurons in the peripheral and central nervous systems, and of other neural crest-derived cell types. Developing sympathetic neurons are absolutely dependent upon nerve growth factor (NGF) during the period of target competition in vivo. Antibodies reacting specifically with NGFR p75 are useful tools in the detection and characterization of NGFR p75, to enhance our understanding of a wide range of phenomena in the development, plasticity and repair of the nervous system.

Specifications

• Antigen: CD271 (NGF Receptor)
• Classification: Monoclonal
• Concentration: 0.5 mg/mL
• Formulation: PBS with 0.09% sodium azide; pH 7.2
• Gene Accession No.: P07174, Q9Z0W1, P08138
• Gene Symbols: Ngfr
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 100349095, 100525607, 101119064, 18053, 24596, 4804, 491071
• Content And Storage: 4° C
• Form: Liquid

• Applications: Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin), Western Blot
• Clone: ME20.4
• Conjugate: Unconjugated
• Gene: NGFR
• Gene Alias: Low-affinity nerve growth factor receptor, NGF receptor, Gp80-LNGFR, p75 ICD, Low affinity neurotrophin receptor p75NTR, p75, LNGFR, p75NTR, RNNGFRR, CD271
• Host Species: Mouse
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Canine, Human, Mouse, Non-human Primate, Ovine, Porcine, Rabbit, Rat
• Product Type: Antibody
• Isotype: IgG1 κ

For Research Use Only.