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Enzo Life Sciences Calcein AM (ultra pure) (1mg). CAS: 148504-34-1
Supplier: Enzo Life Sciences ENZ52002
Calcein acetomethoxy (AM), a derivative of calcein, is useful for differentiating between live and dead cells. Calcein AM readily passes through the cellular plasma membrane. Once inside, esterases cleave the AM groups yielding the more hydrophilic calcein, trapped inside the cell. The loss of the acetomethoxy group also enables calcein to readily bind intracellular calcium; resulting in a strong yellowish-green fluorescence. As dead cells lack cytoplasmic esterases, fluorescence is demonstrated exclusively in live cells, making the probe useful for determining cell viability. Calcein-AM exhibits low cytotoxicity and does not significantly affect cellular functions such as proliferation or chemotaxis. Wavelength Maxima: Excitation 495nm, Emission 515nm. Alternative name: 3',6'-Di(O-acetyl-2',7'-bis[N,N-bis(carboxymethyl)-aminomethyl]fluorescein tetraacetoxymethyl ester. Purity: ≥95% (HPLC). Solubility: Soluble in DMSO. Long Term Storage: -20°C.
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