Invitrogen™ Bovine IL-1 beta, Recombinant Protein

Recombinant Protein

Supplier:  Invitrogen™ RBOIL1BI

Catalog No. ENRBOIL1BI

Description

Bovine IL-1 beta Recombinant Protein, RBOIL1BI, from Invitrogen.

Interleukin-1 beta (IL-1 beta) is a proinflammatory cytokine expressed by monocytes, macrophages, and dendritic cells. IL-1 beta is synthesized in response to inflammatory stimuli as a 31 kDa inactive pro-form that accumulates in the cytosol. Cleavage of pro-IL-1 beta into the active 17 kDa protein requires the activation of inflammasomes, which are multi-protein complexes that respond to pathogens, stress conditions, and other danger signals. Inflammasome activation triggers the processing of the caspase-1 precursor into its active form, which in turn cleaves pro-IL-1 beta. IL-1 beta lacks a signal sequence peptide for classical ER/Golgi pathway and is secreted alongside caspase-1 via an alternate and incompletely understood mechanism. Although IL-1 beta is most often secreted in its active form, secretion of the uncleaved protein may be detectable under some biological conditions. IL-1 beta signals through two receptors, IL-1RI and IL-1RII, both of which are shared with IL-1 alpha. IL-1 beta activity can be moderated by IL-1 Receptor Antagonist (IL-1RA), a protein produced by many cell types that blocks receptor binding through competitive inhibition. IL-1 beta play an important role in innate host defense by triggering the production of other proinflammatory cytokines in target cells and initiating acute-phase responses to infection and injury. Elevated levels of IL-1 beta have been associated with many chronic inflammatory conditions IL-1 beta neutralizing antibodies potential therapeutic value. For dilution, use a buffer containing carrier protein, e.g. PBS with 1% BSA or a neutral media containing 10% FCS and freeze in aliquots at -70°C. Activity: The biological activity of recombinant bovine IL-1-beta was determined by measuring the proliferation of mouse D10.G4.1 cells (Kaye J. et al. (1984) J. Immunol. 133:1339). The ED50 (or 1 unit of activity) for this effect in our laboratories typically falls into the range of 14.1 – 21.1 pg/mL. Expression: Mature bovine IL-1-beta was expressed in Pichia pastoris, KM71H. GenBank™ Accession &Num; P09428, amino acids 114 - 266. For protein verification, the expressed protein was N-terminal sequenced and begins with the following 10 amino acids: *EAEAAPVQSI. *The underlined sequence is due to incomplete cleavage of the alpha-factor secretory signal sequence by the Ste 13 protease. Reconstitute with 0.5 mL of distilled water.

Specifications

• Accession Number: P09428
• Formulation: PBS with no preservative
• Name: Bovine IL-1 beta
• Source: Yeast
• Regulatory Status: RUO
• Common Name: Bovine IL-1 beta
• Specific Reactivity: Bovine
• Cross Reactivity: Bv
• Recombinant: Recombinant
• Protein Subtype: Recombinant

• For Use With (Application): ELISA, Immunoprecipitation, Western Blot
• Gene ID (Entrez): 281251
• Quantity: 20 μg
• Storage Requirements: 4°C
• Gene Alias: Bovine Interleukin 1 beta IL1B, IL-1beta
• Gene Symbol: IL1B
• Conjugate: Unconjugated
• Species: Yeast
• Form: Lyophilized
• Purity or Quality Grade: purified

Safety and Handling

ShelfLife : 12 Months