Abnova™ BCL2 Split CISH Probe

BCL2 Split CISH Probe is designed for the qualitative detection of human BCL2 gene at 18q21.33 in formalin-fixed, paraffin-embedded specimens by chromogenic in situ hybridization (CISH).

Supplier:  Abnova™ CS0002

Catalog No. 89-335-533

Description

The probe is intended to be used in combination with the CISH Implementation Kit 2, which provides necessary reagents for specimen pretreatment and post-hybridization processing.

Hybridization signals of digoxigenin-labeled polynucleotides appear dark green distinct dot-shaped (proximal to the BCL2 breakpoint region), and dinitrophenyl-labeled polynucleotides appear bright red distinct dot-shaped (distal to the BCL2 breakpoint region).
Normal situation: In interphases of normal cells or cells without a translocation involving the BCL2 gene region, two red/green fusion signals appear.
Aberrant situation: One BCL2 gene region affected by a translocation is indicated by one separate green signal and one separate red signal.
Other signal distribution may be observed in some abnormal samples which might result in a different signal pattern than described above, indicating variant rearrangements.
Unexpected signal patterns should be further investigated.

Specifications

• For Use With (Application): CISH-Ce
• Content And Storage: The product is ready-to-use. No reconstitution, mixing, or dilution is required. Bring probe to room temperature (18 to 25°C) and mix briefly before use.