MP Biomedicals™ Basal Medium Eagle (Modified), with Hanks' Salts and L-Glutamine, without Sodium Carbonate

Basal Medium Eagle (Modified), with Hanks' Salts and L-Glutamine, without Sodium Carbonate is one of the most commonly used synthetic basal medium for supporting the growth of many different mammalian cells.

Supplier:  MP Biomedicals™ 091003122

Catalog No. 09-100-3122

Description

• Basal Medium Eagle (BME) is one of the most commonly used of all cell culture media.
• This medium is the result of numerous studies from the late 1950's analyzing the nutrient requirements and other components essential for the growth of cells in culture.
• Traditionally, BME has been used in studies to measure the growth response of normal (WI-38) and transformed (HeLa) cells in monolayer culture.
• BME may be used for a broad variety of cell lines when properly supplemented.
• It has a pH of 6.5 ± 0.5 (w/o sodium bicarb.), 7.3 ± 0.5 (adjusted with sodium bicarb.)

Specifications

• Form: Powder
• For Use With (Application): Bacteria Cell Culture, Mammalian Cell Culture
• Preparation Method: Suspend 42.5 g of the medium in one liter of distilled or deionized water. Mix well and heat with frequent agitation. Boil for one minute. Dispense and sterilize in an autoclave at 121°C (15 lb. psi) for 15 minutes. Cool to 45 to 50°C and add 5 to 10% sterile defibrinated sheep or rabbit blood if desired.

• Formulation: Polypeptone 10.0, Biotryptase 10.0, Heart Peptone 3.0, Corn Starch 1.0, Sodium Chloride 5.0, Agar 13.5, Final pH 7.3 ±0.2
• pH: 6.5 to 7.3 ±0.5
• Quantity: 10 L

Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use.