Applied Biosystems™ High-Capacity cDNA Reverse Transcription Kit

The High Capacity cDNA Reverse Transcription Kit, available with or without RNase Inhibitor, contains all components necessary for the quantitative conversion of up to 2 μg of total RNA into single-stranded cDNA in a single 20 μL reaction.

Supplier:  Applied Biosystems™ 4374967

Catalog No. 43-749-67

Description

The High Capacity cDNA Reverse Transcription Kit, available with or without RNase Inhibitor, contains all components necessary for the quantitative conversion of up to 2 μg of total RNA into single-stranded cDNA in a single 20 μL reaction. Both types of kit deliver extremely high-quality, single-stranded cDNA from 0.02–2 μg total RNA. Reactions can be scaled up to 100 μL to generate 10 μg of cDNA from a single reaction.

The High Capacity cDNA Reverse Transcription Kit with RNase Inhibitor includes the recombinant enzyme used to prevent degradation of RNA templates and lacks DNA endonuclease activity for better product yield.

In addition to their use in RT-PCR, this kit may be used in traditional PCR, microarrays, and for the generation of cDNA archives.

Features of the High Capacity cDNA Reverse Transcription Kit include:
• Linear target amplification for real-time PCR
• Higher yields and precision than other cDNA synthesis kits at a fraction of the cost
• 10-fold greater dynamic range than other kits

Extensively tested with a variety of templates
Quantitative first-strand synthesis of all RNA species is achieved with the use of the random primers. This kit has been extensively tested with a variety of RNA templates, including GC- and AU-rich targets and RNAs expressed at low levels. cRNA can also be efficiently generated from in vitro transcription of cDNA.

Order Info

Shipping Condition: Dry ice

Specifications

• Content And Storage: • 10X RT Buffer (2 x 1 mL)
  • 10X RT Random Primers (2 x 1 mL)
  • 25X dNTP Mix, (1.0 mL at 100 mM)
  • MultiScribe Reverse Transcriptase, (1.0 mL at 50 U/μL)
  • RNase Inhibitor (5 x 200 μL)
  
  Store at -15 to -25°C.
  
  
• Reaction Speed: 120 min.
• Optimal Reaction Temperature: 37°C
• Ribonuclease H Activity: Yes
• For Use With (Application): RT-PCR
• Final Product Type: First-Strand cDNA
• Reaction Format: Separate components
• Size (Final Product): Up to 7 kb
• Includes: Kit with Rnase Inhibitor

• Format: Kit
• Technique: Reverse Transcription
• Reverse Transcriptase: MultiScribe
• Shipping Condition: Dry Ice
• Concentration: 50 U/μL
• No. of Reactions: 1,000 Reactions
• Reagent Type: Reverse Transcription
• Starting Material: RNA

For Research Use Only. Not for use in diagnostic procedures.