Applied Biosystems™ AmpliTaq Gold™ DNA Polymerase with Gold Buffer and MgCl₂

AmpliTaq Gold™ DNA Polymerase is a chemically modified form of AmpliTaq™ DNA Polymerase requiring thermal activation.

Catalog Number	Mfr. No.	Quantity
43-118-06	Applied Biosystems™ 4311806	250 Units
43-118-16	Applied Biosystems™ 4311816	1000 Units
43-118-20	Applied Biosystems™ 4311820	3000 Units
43-118-18	Applied Biosystems™ 4311818	5000 Units
43-177-42	Applied Biosystems™ 4317742	25,000 Units

Description

AmpliTaq Gold™ DNA Polymerase is a chemically modified form of AmpliTaq™ DNA Polymerase requiring thermal activation.

Features of this enzyme:

• Automated chemical hot-start enzyme for increased specificity, sensitivity, and yield
• Time-released thermal activation improves sensitivity in low copy number amplifications
• Successful multiplex PCR saves time and reagents
• Includes GeneAmp™ 10X PCR Gold Buffer with MgCl₂

Hot-start activation

The modified enzyme is provided in an inactive state. Upon thermal activation, the modifier is permanently released, regenerating active enzyme. The resulting hot-start PCR amplification provides increased sensitivity, specificity, and yield over conventional PCR techniques.

Higher specificity, higher yield

AmpliTaq Gold™ DNA Polymerase can be activated partially or completely in a pre-PCR heat step, or can be allowed to activate slowly in a time-released manner during the denaturation steps of thermal cycling. With or without a limited up-front heat activation step, active enzyme is released slowly during thermal cycling to match template concentration and increase specificity. The yield of specific product increases because reactants are not wasted in the formation of unintended products. Because AmpliTaq Gold™ DNA Polymerase is a chemical hot-start enzyme, there is no worry of biological contamination.

GeneAmp™ 10X PCR Gold Buffer is formulated to provide flexible, efficient activation of AmpliTaq Gold™ DNA Polymerase, resulting in a highly specific, robust PCR amplification. The ionic strength and the pH of GeneAmp™ 10X PCR Gold Buffer have been optimized to provide a wider activation temperature and time range when used in conjunction with AmpliTaq Gold™ DNA Polymerase.

AmpliTaq Gold™ 360 DNA Polymerase for superior performance

Also available is AmpliTaq Gold™ 360 DNA Polymerase for even better PCR performance. Compared to AmpliTaq Gold™ DNA Polymerase, AmpliTaq Gold™ 360 DNA Polymerase is purified by an additional proprietary separation process to eliminate contaminating bacterial DNA sequences from the enzyme preparation. When used with the enhanced 360 Gold Buffer, this ultra-pure enzyme, in addition to its hot-start capabilities, reduces false positive results, amplifies low-level target sequences, and promotes the amplification of a variety of templates. Also included with this product is a 360 GC Enhancer to work through challenging GC-rich templates.

Order Info

Shipping condition: Dry Ice

Specifications

• GC-Rich PCR Performance: Low
• Exonuclease Activity: 5' - 3'
• Concentration: 5 U/μL
• Hot Start: Built-In Hot Start
• Reaction Format: Separate Components

• Polymerase: AmpliTaq Gold DNA Polymerase
• Product Type: DNA Polymerase
• Fidelity (vs. Taq): 1X
• Overhang: 3'-A
• Size (Final Product): 5 kb or less

For Research Use Only. Not for use in diagnostic procedures.