Applied Biosystems™ AmpliTaq Gold™ DNA Polymerase with Buffer II and MgCl₂

AmpliTaq Gold™ DNA Polymerase is a chemically modified form of AmpliTaq™ DNA Polymerase requiring thermal activation.

Supplier:  Applied Biosystems™ N8080247

Catalog No. N8080247

Description

AmpliTaq Gold™ DNA Polymerase is a chemically modified form of AmpliTaq™ DNA Polymerase requiring thermal activation.

Features of this enzyme:

• Automated chemical hot-start enzyme for increased specificity, sensitivity, and yield
• Time-released thermal activation improves sensitivity in low copy number amplifications
• Successful multiplex PCR saves time and reagents
• Includes GeneAmp™ 10X PCR Buffer II with MgCl₂

Hot-start activation

The modified enzyme is provided in an inactive state. Upon thermal activation, the modifier is permanently released, regenerating active enzyme. The resulting hot-start PCR amplification provides increased sensitivity, specificity, and yield over conventional PCR techniques.

Higher specificity, higher yield

AmpliTaq Gold™ DNA Polymerase can be activated partially or completely in a pre-PCR heat step, or can be allowed to activate slowly in a time-released manner during the denaturation steps of thermal cycling. With or without a limited up-front heat activation step, active enzyme is released slowly during thermal cycling to match template concentration and increase specificity. The yield of specific product increases because reactants are not wasted in the formation of unintended products. Because AmpliTaq Gold™ DNA Polymerase is a chemical hot-start enzyme, there is no worry of biological contamination.

AmpliTaq Gold™ 360 DNA Polymerase for superior performance

Also available is AmpliTaq Gold™ 360 DNA Polymerase for even better PCR performance. Compared to AmpliTaq Gold™ DNA Polymerase, AmpliTaq Gold™ 360 DNA Polymerase is purified by an additional proprietary separation process to eliminate contaminating bacterial DNA sequences from the enzyme preparation. When used with the enhanced 360 Gold Buffer, this ultra-pure enzyme, in addition to its hot-start capabilities, reduces false positive results, amplifies low-level target sequences, and promotes the amplification of a variety of templates. Also included with this product is a 360 GC Enhancer to work through challenging GC-rich templates.

Order Info

Shipping condition: Dry Ice

Specifications

• Content And Storage: • AmpliTaq Gold DNA Polymerase (5 U/μL), 200 μL
  • 10X PCR Buffer II, 4 x 1.5 mL
  • 25 mM MgCl₂, 4 x 1.5 mL
  
  Store at -15°C to -30°C.
• GC-Rich PCR Performance: Low
• Reaction Speed: Standard
• Product Type: DNA Polymerase
• Shipping Condition: Dry Ice
• Concentration: 5 U/μL
• Hot Start: Built-In Hot Start
• Overhang: 3'-A
• Size (Final Product): 5 kb or less

• Format: Tube
• Polymerase: AmpliTaq Gold DNA Polymerase
• Exonuclease Activity: 5' - 3'
• Quantity: 1000 Units
• For Use With (Application): Hot-start PCR
• Fidelity (vs. Taq): 1X
• No. of Reactions: 800 Reactions
• Reaction Format: Separate Components
• Starting Material: DNA

For Research Use Only. Not for use in diagnostic procedures.