Invitrogen™ PureLink™ RNA Micro Scale Kit

Provides rapid purification and concentration of total RNA from a variety of samples (e.g., LCM tissues, fine needle aspirates, and FACS-sorted cells)

Supplier:  Invitrogen™ 12183016

Catalog No. 12-183-016

Description

A simple and reliable technology for everyday RNA purification
PureLink™ technology combines guanidine-isothiocyanate lysis with the speed, purity, and ease-of-use of silica-membrane purification. The safe and easy procedure can be completed in typically less than 15 minutes with >85% recovery from the first elution. The kit can be used for clean-up of purified RNA from enzymatic reactions, desalting of RNA samples after phenol extraction or ethanol precipitation, and concentration of RNA. Purify RNA from up to 10mg of tissue or 10⁵ cells. Clean up and concentrate RNA amounts ranging from pg to 100μg in volumes as small as 10μL. Purified RNA is ready for use in downstream applications such as RT-PCR, qRT-PCR, northern blots, cDNA library construction, and nuclease protection assays.

A simple and rapid procedure
Homogenize your sample in lysis buffer, add 70% ethanol, and mix. Deposit the resulting solution into a PureLink Micro kit column with collection tube, and centrifuge for one minute. Nucleic acids bind to the PureLink silica membrane in the column, and after subsequent washes and on-column digestion with DNase, purified RNA can be eluted with RNase-free water.

• Effective: Expect excellent RNA recovery (up to 100μg total RNA) from small samples
• Convenient and safe: The extraction procedure uses no phenol/chloroform, CsCl gradients, or precipitation with either LiCl or ethanol
• Fast: Obtain high-quality total RNA, typically in just 15 minutes
• Reliable: Obtain ready-to-use RNA suitable for any downstream application

Specifications

• Content And Storage: Box 1 (PureLink™ RNA Micro Kit):
  • Lysis Buffer, 125 mL
  • Wash Buffer I, 50 mL
  • Wash Buffer II, 15 mL
  • RNase-free Water, 15.5 mL
  • PureLink™ Micro Kit Columns with collection tubes, 50 each
  • Collection Tubes, 50 each
  • Recovery Tubes, 50 each
  
  Box 2 (PureLink™ DNase/Carrier RNA):
  • Lyophilized PureLink™ DNase, 1500 Units
  • PureLink™ On-Column DNase 2X Buffer, 1 mL
  • RNase-free Water, 1 mL
  • Lyophilized PureLink™ Carrier RNA, 300 μg
  
  Store all components of Box 2 at 4°C. Store remaining components at room temperature.
• Purification Time: 15 min. (After sample homogenization)
• Sample Type: Cells, Tissue, Laser Capture Microdissection (LCM), Enzymatic Reactions
• High-throughput Compatibility: Not High-throughput Compatible (Manual)
• Quantity: 50 Preps
• Shipping Condition: Room Temperature
• Yield: Up to 17 μg

• Isolation Technology: Silica Spin Column
• Elution Volume: 12–22 μL
• Final Product Type: Total RNA
• For Use With (Application): RT-PCR, qPCR, cDNA Library Construction, NGS, Microarray Analysis, Blot Hybridization, Northern Blotting, In Vitro Translation, Nuclease Protection Assays, Nucleic Acid Labeling
• Scale: Micro
• Starting Material Amount: Cells: ≤10⁵
  Tissue: ≤5 mg
  Enzymatic Reactions: ≤1.2 mL

For Research Use Only. Not for use in diagnostic procedures.