Invitrogen™ NorthernMax™-Gly Kit

An alternative to formaldehyde-based systems for northern analysis and uses a glyoxal/DMSO Loading Solution for sample denaturation

Supplier:  Invitrogen™ AM1946

Catalog No. AM1946

Includes:

Positive Control RNA, pTRI-GAPDH Mouse, DECAtemplate §-actin-Mouse, Formaldehyde Load Dye, ULTRAhyb Buffer, 10X Denaturing Gel Buffer, and 10X MOPS Gel Running Buffer, Agarose-LE, Transfer Buffer, Low Stringency Wash Solution No. 1, High Stringency Wash Solution No. 2, and RnaseZap™, nuclease-free water

Description

• Use of glyoxal/DMSO instead of formaldehyde for sample preparation avoids the need to pour and run gels in a fume hood, and eliminates the safety issues associated with use of formaldehyde
• Glyoxal gels can be prepared at the lab bench: ;no noxious reagents
• Increased sensitivity up to 100x over standard hybridization protocols
• Reduce hybridization to 2 hours for many messages
• Complete kit contains ULTRAhyb™ Hybridization Buffer and gel and wash reagents for 10 to 20 gels
• Compatible with RNA, DNA, or oligonucleotide probes labeled isotopically or nonisotopically
• Sufficient reagents are provided for processing 1000cm² membrane
• RNA samples denatured with glyoxal may show sharper bands on northern blots compared to samples denatured and run in the presence of formaldehyde
• The streamlined NorthernMax-Gly procedure is more convenient and faster than the standard glyoxal gel protocol: A single reagent is used for sample denaturation and loading, the incubation time for sample denaturation has been reduced, and no recirculation of buffer is required during electrophoresis
• The volume ratio of glyoxal denaturation solution to sample RNA is lower than many protocols, so that sample precipitation prior to gel loading is usually not required
• An RNA sample and template for probe synthesis are also provided as controls for each kit
• Compatible with glyoxal-containing gels

DNA and RNA Purification and Analysis, Northern Blotting, Nucleic Acid Gel Electrophoresis and Blotting

Order Info

Shipping Condition: Room Temperature

Specifications

• Content And Storage: • Positive Control RNA, pTRI-GAPDH Mouse, DECAtemplate §-actin-Mouse, and Glyoxal Load Dye should be stored at -20°C.
  • ULTRAhyb™ Buffer and 10X Gel Prep⁄Running Buffer should be stored at 4°C.
  • Agarose-LE™, Transfer Buffer, Low Stringency Wash Solution #1, mL High Stringency Wash Solution #2, and RNaseZap™ should be stored at room temperature.
  • Nuclease-free water may be stored at any temperature.
  
• Gel Compatibility: Glyoxal-Containing Gels
• Shipping Condition: Room Temperature

• Product Type: Northern Analysis Reagents
• Quantity: 1 kit

For Research Use Only. Not for use in diagnostic procedures.