Invitrogen™ MEGAscript™ T3 Transcription Kit

Synthesize 10 to 50 times the amount of RNA produced by conventional transcription reactions

Supplier:  Invitrogen™ AM1338

Catalog No. AM1338

Description

Novel transcription reaction conditions and Ambion's patented, high-yield technology allow the synthesis of 10 to 50 times the amount of RNA produced by conventional transcription reactions. Each Ambion kit includes sufficient reagents for 40 reactions.

• Exclusive, ultrahigh yield technology is fast: 2 hour reaction time
• Amplifies aRNA for gene array analysis and other applications
• Efficiently incorporates many modified nucleotides
• A typical 20μL MEGAscript reaction with 1μg of the pTRI-Xef-1α control template will yield over 100μg of transcript
• Versatility of the MEGAscript kit allows for manipulation of the reactions to include specialized reagents such as modified nucleotides, cap analog, or additional polymerase

Accessory Products:
MessageAmp™ aRNA Amplification Kits are powered by MEGAscript technology and have been developed especially for the amplification of cRNA prior to array analysis. To purify in vitro transcription products from free nucleotides, buffer components, and enzymes, use MEGAclear™ Kit (Mfr. No. AM1908) and the MEGAclear-96 Kit (Mfr. No. AM1909).

Specifications

• Content And Storage: T3 Enzyme Mix, 10X Reaction Buffer, ATP Solution, CTP Solution, GTP Solution, UTP Solution, pTRI-Xef, TURBO DNase, Ammonium Acetate Stop Solution, Lithium Chloride Precipitation Solution, and Gel Loading Buffer II are all stored at –20°C. Nuclease-free Water may be stored at any temperature.
• Format: Kit
• For Use With (Application): In Vitro Transcription, synthetic mRNA, mRNA, transfection, LNPs, GENEart, linearized DNA plasmid, delivery, PCR products
• Product Line: Ambion™, MEGAscript™
• Promoter: T3
• Yield: 100 μg

• Final Product Type: Synthetic mRNA, mRNA
• Sample Type: mRNA, Synthetic mRNA
• No. of Reactions: 40 Reactions
• Product Type: T3 In Vitro Transcription kit
• Shipping Condition: Dry Ice
• Starting Material: PCR Products, Linearized DNA plasmid

For Research Use Only. Not for use in diagnostic procedures.