Invitrogen™ MAXIscript™ T7 Transcription Kit

Fast synthesis of RNA probes for use in ribonuclease protection assays, in situ hybridization, and blot hybridizations

Includes: T7 Enzyme Mix, 10X Transcription Buffer, ATP Solution, CTP Solution, GTP Solution, UTP Solution, TURBO DNase, Gel Loading Buffer II, pTRI-b-actin, Nuclease-free Water

Catalog Number	Mfr. No.	Product Type	Quantity
AM1312	Invitrogen™ AM1312	T7 In Vitro Transcription kit	30 reactions
AM1314	Invitrogen™ AM1314	T7 In Vitro Transcription kit	100 Reactions
AM1314M	Invitrogen™ AM1314M	T7 In Vitro Transcription kit	100 Reactions

Description

The Ambion™ MAXIscript™ T7 In Vitro Transcription Kit synthesizes RNA probes in just 10 minutes for use in ribonuclease protection assays, in situ hybridization, and blot hybridizations. Each kit contains sufficient reagents for 30 reactions. The MAXIscript In Vitro Transcription Kit synthesizes RNA probes with specific activities reaching 1 × 10⁹ cpm/μg in just 10 minutes. MAXIscript Kits are very efficient at synthesizing full-length probes, even at limiting nucleotide concentrations, making them the perfect companion to ribonuclease protection assays. MAXIscript Kits are also an excellent choice for the synthesis of nonisotopically labeled probes. Labeling can be accomplished post-transcriptionally by using Ambion's BrightStar™ Psoralen-Biotin Labeling Kit, or during transcription by incorporating modified nucleotides into the reaction. MAXIscript Kits can also be used for the synthesis of modest amounts of unlabeled RNA (2–6μg/20μL reaction) of up to 2kb.

• High yields of high specific activity, full-length RNA probes in just 10 minutes
• Ideal probe synthesis kit for nuclease protection assays, Northern and Southern blots, and in situ hybridization
• Includes TURBO DNase™
• All kits contain quality-tested DNase/RNase-free reagents
• RNA Yield: 2 to 6μg

Specifications

• Content And Storage: T7 Enzyme Mix, 10X Transcription Buffer, ATP Solution, CTP Solution, GTP Solution, UTP Solution, TURBO DNase, Gel Loading Buffer II, and pTRI-b-actin are all stored at –20°C. Nuclease-free Water may be stored at any temperature.
• Format: Kit
• For Use With (Application): In Vitro Transcription, synthetic mRNA, mRNA, transfection, LNPs, GENEart, linearized DNA plasmid, delivery, PCR products
• Product Type: T7 In Vitro Transcription kit
• Yield: 2-6 μg

• Final Product Type: Synthetic mRNA, mRNA
• Sample Type: mRNA, Synthetic mRNA
• Product Line: Ambion™, MAXIscript™
• Promoter: T7
• Starting Material: PCR Products, Linearized DNA plasmid

For Research Use Only. Not for use in diagnostic procedures.