Invitrogen™ mirVana™ miRNA Isolation Kit, without phenol

The mirVana™ miRNA Isolation Kit uses a rapid procedure to isolate small RNAs from tissue and cells using an efficient glass fiber filter (GFF)-based method.

Supplier:  Invitrogen™ AM1561

Catalog No. AM1561

Description

The mirVana™ miRNA Isolation Kit uses a rapid procedure to isolate small RNAs from tissue and cells using an efficient glass fiber filter (GFF)-based method. The method isolates total RNA ranging in size from kilobases down to 10-mers. Each kit contains sufficient reagents and consumables for either 40 isolations of total RNA (including small RNAs), or 20 separate large and small RNA fractions. Phenol must be purchased separately. Features of this kit:

• Efficient isolation of small RNA-containing total RNA
• Enrich for small RNA (less than 200 nt) to increase sensitivity in downstream analyses
• Simple 30 minute procedure
• Ideal for miRNA, siRNA, shRNA, and snRNA analysis
• Compatible with virtually all cell and tissue types

Efficient recovery of small RNA
Current RNA purification procedures rely on organic extraction, followed by alcohol precipitation or adsorption of nucleic acid molecules on a GFF or silicate matrix. Unfortunately, the former procedure requires alcohol precipitation, which is time consuming and inefficiently recovers small RNAs. The latter procedure typically results in the loss of substantial amounts of small RNA. The mirVana™ miRNA Isolation Kit uses organic extraction followed by purification on a GFF under specialized binding and wash conditions. As a result, the kit effectively recovers all RNA—from large mRNA and ribosomal RNA down to 10-mers—from virtually all cell and tissue types. The mirVana™ miRNA Isolation Kit enables the isolation of small RNA-containing total RNA from samples consisting of 10²–10⁷ cultured cells or 0.5–250 mg tissue.

Enrich for small RNA
Although the mirVana™ miRNA Isolation Kit efficiently purifies all RNA larger than 10 nt, it also includes procedures for isolating RNA fractions, specifically enriched or depleted in small RNA species. Through the enrichment procedure included in the mirVana™ miRNA Isolation Kit, RNA molecules of ∼200 nt and less can be efficiently purified away from the larger RNA species. The resulting RNA preparation is highly enriched for miRNAs, siRNAs, and/or snRNAs. Small RNA enrichment using the mirVana™ miRNA Isolation Kit procedure allows more sensitive small RNA detection with less background as compared to the same assay used with total RNA.

Specifications

• Content And Storage: • 30 mL miRNA Wash Solution I (room temperature)
  • 50 mL Wash Solution 2/3 (room temperature)
  • 80 Collection Tubes (room temperature)
  • 40 Filter Cartridges (room temperature)
  • 100 mL Lysis/Binding Buffer (4°C)
  • 10 mL miRNA Homogenate Additive (4°C)
  • 1.4 mL Gel Loading Buffer II (-20°C)
  • 5 mL Elution Solution (-20°C, 4°C, or room temperature)
• Purification Time: 30 min. (After sample homogenization)
• Sample Type: Bacteria, Cells, Plant, Tissue, Viruses, Yeast, Enzymatic Reactions
• High-throughput Compatibility: Not High-throughput Compatible (Manual)
• Quantity: 40 Preps
• Starting Material Amount: Bacteria: ≤10⁷ cells
  Cells: ≤10⁷
  Plant: ≤250 mg
  Tissue: ≤250 mg
  Virus: ≤10⁷ cells
  Yeast: ≤10⁷ cells
  Enzymatic Reactions: varies

• Isolation Technology: Spin Column (Glass Fiber Filter), Organic Extraction
• Elution Volume: 100 μL
• Final Product Type: Small RNA
• For Use With (Application): qRT-PCR, microarray analysis, Northern blotting
• Shipping Condition: Room Temperature
• Yield: Cells: ≤16 μg RNA per 50,000 cells
  Tissue: ≤160 μg per 5 mg

For Research Use Only. Not for use in diagnostic procedures.