Invitrogen™ FirstChoice™ RLM-RACE Kit

Designed to amplify cDNA only from full-length, capped mRNA, usually producing a single band after PCR

Supplier:  Invitrogen™ AM1700

Catalog No. AM1700

Includes: Exo– Klenow, 10X Decamer Solution, 5X Reaction Buffer minus dATP, 5X Reaction Buffer minus dCTP, DECAtemplate GAPDH-M, and 0.5M EDTA should be stored at –20°C. Nuclease-free water may be stored at any temperature.

Description

The FirstChoice RLM-RACE Kit is a major improvement over the basic rapid amplification of cDNA ends (RACE) protocol. The RLM-RACE procedure selects only full-length mRNA—no rRNA, tRNA or degraded RNA—and facilitates the cloning of sequences from the 5' ends of messages.

• From RNA to PCR product in less than a day
• Yields single, specific product from rare transcripts
• Selects 5' and/or 3' ends of true messages
• Efficient—
• Al enzymatic reactions are optimized to ensure detection of even rarest mRNA
• Final product size: 7kb or less
• Optimal reaction temperature: 42°C
• Sample: RNA

• Rapid amplification of cDNA ends (5'-RACE) is polymerase chain reaction-based technique developed to facilitate cloning of sequence from the 5'-ends of messages
• Improvement to basic RACE protocol
• Selects full-length mRNA—no rRNA, tRNA or degraded mRNA

Optimal reaction temperature: 42°C

Cloning, PCR and Real-Time PCR, Reverse Transcription, cDNA Libraries and Library Construction

Order Info

Shipping Condition: Dry ice

Specifications

• Product Type: RLM-RACE Kit
• Content And Storage: The following components should be stored at –20°C:
  • Calf Intestine Alkaline Phosphatase
  • 10X CIP Buffer
  • Tobacco Acid Pyrophosphatase
  • 10X TAP Buffer, T4 RNA Ligase
  • 10X T4 RNA Ligase Buffer
  • 5' RACE Adapter
  • 3' RACE Adapter
  • 5' RACE Outer Primer
  • 5' RACE Inner Primer
  • 3' RACE Outer Primer
  • 3' RACE Inner Primer
  • Ammonium Acetate Stop Solution
  • M-MLV Reverse Transcriptase
  • dNTP Mix
  • 10X RT Buffer
  • 10X PCR Buffer
  • Random Decamers
  • RNase Inhibitor
  • Mouse Thymus RNA
  • 5' RACE Outer Control Primer
  • 5' RACE Inner Control Primer
  • 5' PCR Control Primer
  • 3' RACE Control Primer
  Nuclease-free water may be stored at any temperature.
  
• Optimal Reaction Temperature: 42°C
• Shipping Condition: Dry Ice
• Product Line: Ambion™, FirstChoice™
• Quantity: 1 Kit

• Includes: Exo– Klenow, 10X Decamer Solution, 5X Reaction Buffer minus dATP, 5X Reaction Buffer minus dCTP, DECAtemplate GAPDH-M, and 0.5 M EDTA should be stored at -20°C. Nuclease-free water may be stored at any temperature.
• Final Product Type: cDNA
• Reverse Transcriptase: M-MLV
• Format: Kit
• For Use With (Application): cDNA Libraries & Library Construction

For Research Use Only. Not for use in diagnostic procedures.