Abnova™ ALK Split CISH Probe

ALK Split CISH Probe is designed for the qualitative detection of human ALK gene at 2p23.2 in formalin-fixed, paraffin-embedded specimens by chromogenic in situ hybridization (CISH).

Supplier:  Abnova™ CS0001

Catalog No. 89-335-532

Description

The probe is intended to be used in combination with the CISH Implementation Kit 2, which provides necessary reagents for specimen pretreatment and post-hybridization processing.

Hybridization signals of digoxigenin-labeled polynucleotides appear dark green distinct dot-shaped (proximal to the ALK breakpoint region), and dinitrophenyl-labeled polynucleotides appear bright red distinct dot-shaped (distal to the ALK breakpoint region).
Normal situation: In interphases of normal cells or cells without a translocation involving the ALK gene region, two red/green fusion signals appear.
Aberrant situation: One ALK gene region affected by a translocation is indicated by one separate green signal and one separate red signal. EML4-ALK inversion with deletion of 5'-ALK sequences is indicated by one or multiple isolated red signals.
Other signal distribution may be observed in some abnormal samples which might result in a different signal pattern than described above, indicating variant rearrangements.
Unexpected signal patterns should be further investigated.

Specifications

• Content And Storage: The product is ready-to-use. No reconstitution, mixing, or dilution is required. Bring probe to room temperature (18 to 25°C) and mix briefly before use.
• For Use With (Application): CISH-P