Molecular Probes™ Alexa Fluor™ 488 Streptavidin, 5 nm colloidal gold conjugate

Alexa Fluor™ 488 Streptavidin, 5 nm colloidal gold conjugate

Supplier:  Molecular Probes™ A32360

Catalog No. A32360

Description

Alexa Fluor™ 488 Streptavidin comprises a biotin-binding protein (streptavidin) covalently attached to a fluorescent label (Alexa Fluor™ dye). Streptavidin has a very high binding affinity for biotin, and a conjugate of streptavidin is commonly used together with a conjugate of biotin for specific detection of a variety of proteins, protein motifs, nucleic acids, and other molecules (for example, a biotinylated primary antibody bound to a protein target can be detected with a fluorescently labeled streptavidin). Strategies similar to this are used in many detection protocols including western blots, flow cytometry, imaging and microscopy, and microplate assays. Alexa Fluor™ dye streptavidin conjugates are supplied as 1 mg lyophilized product or in 0.5 mL volumes of a 2 mg/mL solution.

Important Features of Alexa Fluor™ 488 Streptavidin Conjugates:

Alexa Fluor™ 488 streptavidin conjugate has Ex/Em maxima of ∼ (495/519)

Bright, photostable fluorescence

High solubility in aqueous solutions

Available in multiple colors

Ideal for western blots, flow cytometry, imaging and microscopy, microplate assays and more

Properties of Alexa Fluor™ Dyes
Alexa Fluor™ dyes are organic fluorescent dyes developed for better performance in imaging and other labeling protocols and exhibit improved photostability and brightness and improved solubility in aqueous solutions. Available in a broad range of colors, these dyes are a good choice for most imaging applications.

Blocking Endogenous Biotin
Naturally occurring biotins can interfere with biotin-streptavidin detection schemes. For experiments involving fixed and permeabilized cells, try our Endogenous Biotin-Blocking Kit to minimize this interference.

For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.

Specifications

• Concentration: 30 μg/mL
• Content And Storage: Store in refrigerator (2–8°C) and protect from light.
• Form: Liquid
• Product Type: Streptavidin Conjugate (fluorescent)

• Conjugate: Alexa Fluor 488, FluoroNanogold
• Shipping Condition: Wet Ice
• Product Line: Alexa Fluor™
• Label or Dye: Alexa Fluor Dyes

For Research Use Only. Not for use in diagnostic procedures.