Invitrogen™ AccuPrime™ SuperMix I

AccuPrime SuperMix I is a ready-to-use mixture of DNA polymerase, accessory proteins, salts, magnesium, and dNTPs for efficient PCR amplification.

Supplier:  Invitrogen™ 12342010

Catalog No. 12-342-010

Description

AccuPrime SuperMix I is a ready-to-use mixture of DNA polymerase, accessory proteins, salts, magnesium, and dNTPs for efficient PCR amplification. All you have to do is add template and primers, reducing set-up time by half. For accurate PCR performance, AccuPrime SuperMix I sets the standard. You'll amplify your DNA targets cycle after cycle, eliminating troubleshooting and rework.

In addition to a powerful DNA polymerase, AccuPrime SuperMix I includes Platinumanti-DNA polymerase antibodies that inhibit polymerase activity, providing an automatic hot-start, and a thermal stable accessory protein that enhances specific primer-template hybridization during every cycle of PCR. This unique combination allows for success, even under previously suboptimal conditions.

AccuPrime SuperMix I is designed for amplification of genomic DNA amplicons (> 200 bp), plasmid DNA, or cDNA templates. Alternatively, AccuPrime SuperMix II is designed to amplify a broader range of amplicon sizes up to 4 kb.

• Built-in hot start

Order Info

Shipping Condition: Dry Ice

Specifications

• Content And Storage:

  AccuPrime SuperMix I is supplied at 2X concentration to allow approximately 50% of the final reaction volume to be used for the addition of primer and template solutions. Reagents sufficient for 200 amplification reactions of 25 μL each are provided. AccuPrime SuperMix I Components: 40 mM Tris-HCl (pH 8.4), 100 mM KCl, 3 mM MgCl₂, 400 μM dGTP, 400 μM dATP, 400 μM dTTP, 400 μM dCTP, AccuPrime Taq DNA Polymerase, thermostable AccuPrime protein, stabilizers.
  
  200 Reactions: 2 x 1.25 mL.
  Store at -20°C in a non-frost-free freezer.

• For Use With (Application): Hot-start PCR, High-fidelity PCR
• No. of Reactions: 200 Reactions

• Gel Compatibility: Low
• Fidelity (vs. Taq): 2X

For Research Use Only. Not for use in diagnostic procedures.